Table primers ued for pcr and direct sequencing




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Table S2. Primers ued for PCR and direct sequencing.


Gene region

Primer name

Primer sequence

Reference

18S rRNA (18S)


18S1F (forward)


5’-TAC CTG GTT GAT CCT GCC AGT AG-3’


Cryan et al. (2004)






18SR1cicada (reverse)

5’-ACC GAG GTC CTA TTC CAT TA-3’

this study

Wingless (Wg)

LepWG1 (forward)

5’-GAR TGY AAR TGY CAY GGY ATG TCT GG-3’

Brower & DeSalle (1998)




ModLepWG2 (reverse)

5’-ACT NCG CAR CAC CAR TGG AAT GTR CA-3’

Brower & DeSalle (1998)

Elongation factor 1-alpha (EF1-a)

EF1a-97-for (forward)

5’-ACG CCC CTG GAC ATA GAG AT-3’

Buckley et al. (2006)

EF1a-352-rev (reverse)

5’-TGT GTA GCC ATT TGA AAT TTG G-3’

Buckley et al. (2006)

EF1-PA-f650mod (forward)

5’-TGC TGC GGG TAC TGG TGA AT-3’

Sueur et al. (2007)

EF1A189R2 (reverse)

5’-CAR CCT GAG ATT GGC ACA AA-3’

modified from Buckley et al. (2006)

Calmodulin (Cal)

Cal-60-for (forward)

5’-AAC GAA GTA GAT GCC GAT GG-3’

Buckley et al. (2006)

Cal_magR3 (reverse)

5’-CAA CCT ATT CTA AAA TTC ATC AAT TGG C-3’

this study

Cytochrome oxidase subunit I-II (COI-II)

COS1751magicicada (forward)

5’-GGT GCT CCY GAY ATR GCY TTY CC-3’

modified from C1-J-1751; Simon et al. (1994)

C2-N-3661 cicada (reverse)

5’-CCA CAA ATT TCA GAA CAT TGA CCA-3’

Simon et al. (1994)

COcassiniL1 (internal)

5’-CCT CCG TCT GAG CAC TCA TAT AGT G-3’

this study

COcassiniE1 (internal)

5’-CGT GTA TCA ACA TCT ATA CCA ACC-3’

this study

COcassiniE2 (internal)

5’-GAA ATT CTT CTT CCA ATA GAA G-3’

this study

COcassiniL2 (internal)

5’-GAT TAT ATT TGT TGG AGG TAA A-3’

this study

References: Cryan JR, et al. (2004) Treehopper trees: phylogeny of Membracidae (Hemiptera: Cicadomorpha: Membracoidea) based on molecules and morphology. Syst Entomol 29: 441-454; Brower AZW, DeSalle R (1998) Patterns of mitochondrial versus nuclear DNA sequence divergence among nymphalid butterflies: the utility of wingless as a source of characters for phylogenetic inference. Insect Mol Biol 7: 73-82; Buckley TR, Cordeiro M, Marshall DC, Simon C (2006) Differentiating between hypotheses of lineage sorting and introgression in New Zealand alpine cicadas (Maoricicada Dugdale). Syst Biol 55: 411-425; Sueur J, et al. (2007) Molecular phylogeny of the genus Tibicina (Hemiptera, Cicadidae): rapid radiation and acoustic behaviour. Biol. J. Linn. Soc. 91: 611-626; Simon C, et al. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87: 651-701.








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