Table isolates used as negative controls in both TaqMan




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TABLE S3. Isolates used as negative controls in both TaqMan® PCR systems.

Species

Strain

Location

aPCR results

P. chlamydospora

P. aleophilum

P. chlamydospora


*bCBS161.90

Italy

+

-

P. chlamydospora

cLR47

Hérault, France

+

-

P. chlamydospora

dINIA56b

Madrid, Spain

+

-

P. chlamydospora

Y87-9-1b

Arribes Duero, Spain

+

-

P. chlamydospora

Y121-2-91b

Arribes Duero, Spain

+

-

P. aleophilum

•bCBS631.94

Italy

-

+

P. aleophilum

Y38-5-3b

Valle Benavente, Spain

-

+

P. aleophilum

Y69-6-1b

Ribera Duero, Spain

-

+

P. aleophilum

Y86-15-1b

Toro, Spain

-

+

P. aleophilum

Y108-2-2a

Extremadura, Spain

-

+

P. scolyti

*bCBS113593

France

-

-

P. viticola

•*bCBS113065

South Africa

-

-

P. viticola

Y271-3-1d

Ribera Duero, Spain

-

-

P. hispanicum

Y549-9-3b

Nursery, Spain

-

-

P. iranianum

Y277-14-1

Rioja Alabesa, Spain

-

-

P. mortoniae

Y270-16-1

Ribera Duero, Spain

-

-

Cylindrocarpon destructans

•*bCBS119.41

Netherlands

-

-

C. macrodidymum

•bCBS120170

Slovenia

-

-

C. macrodidymum

Y98-3-2a

Cigales, Spain

-

-

C. liriodendri

*VR37-1

Nursery, Spain

-

-

C. liriodendri

Y99-4-1c

Cigales, Spain

-

-

C. olidum

Y112-34-1c

Arribes Duero, Spain

-

-

C. olidum

Y160-57-1a

La Mancha, Spain

-

-

C. pauciseptatum

e S20-2-2a

Salamanca, Spain

-

-

Diplodia seriata

•*bCBS112555

Portugal

-

-

Di. seriata

bCBS719.85

New Zealand

-

-

Di. seriata

Napa-a

California, USA

-

-

Di. mutila

•*bCBS431.82

Netherlands

-

-

Botryosphaeria dothidea

•*bCBS110302

Portugal

-

-

B. dothidea

Sonoma

California, USA

-

-

Dothiorella sarmentorum

•*bCBS120.41

Norway

-

-

Do. sarmentorum

Y129-4-1

Nursery, Spain

-

-

Do. iberica

•*bCBS115035

Spain

-

-

Do. iberica

Y81-1-2

Ribera Duero, Spain

-

-

Do. viticola

•*bCBS117006

Spain

-

-

Neofusicoccum parvum

•bCBS110301

Portugal

-

-

N. parvum

dINIA352

Madrid, Spain

-

-

N. luteum

•*bCBS110299

Portugal

-

-

N. australe

Y264-21-1

Nursery, Spain

-

-

N. ribis

*bCBS655.77

New Zealand

-

-

Lasiodiplodia theobromae

*Y501-3-5a

Nursery, Spain

-

-

Phomopsis viticola

*bCBS267.80

Italy

-

-

Cryptovalsa ampelina

*Y231-5-4

Ribera Duero, Spain

-

-

Stereum hirsutum

•*bCBS416.61

Austria

-

-

S. hirsutum

Y112-29-1

Arribes Duero, Spain

-

-

Eutypa lata

•*bCBS101932

Netherlands

-

-

E. lata

Jerez-a

Jerez Frontera, Spain

-

-

E. lata

Y249-4-1b

Toro, Spain

-

-

Fomitiporia mediterranea

Y189-22-2b

Cigales, Spain

-

-

F. mediterranea

*Y172-4-1

Alicante, Spain

-

-

Fusarium solani.

*Y134-3-1a

Nursery, Spain

-

-

Alternaria solani

•*bCBS109157

USA

-

-

Trichoderma sp

fNBT

Sevilla, Spain

-

-

Helminthosporium solani

bCBS640.85

The Netherlands

-

-

Rhizoctonia solani

bCBS163.83

The Netherlands

-

-

Colletotricum acutatum

*fImi

Salamanca, Spain

-

-

Vitis vinifera

Tempranillo

Valladolid, Spain

-

-

Vitis vinifera

Garnacha

Valladolid, Spain

-

-

Vitis vinifera

Viura

Valladolid, Spain

-

-

* Isolates used in SYBR-Green specificity testing with the palFI1/palRI1 primers.

Isolates used in SYBR-Green specificity testing with the pchITS417F/pchITS495R primers.

a TaqMan® PCR results using primers described in this work.

b Isolates supplied by Centraalbureau voor Schimmelcultures Uppsalalaan, Utrecht, The Netherlands.

c Isolate supplied by Dr. J-P. Péros, INRA, Villeneuve-les-Maguelone, France.

d Isolates supplied by Dr. R. Raposo, Instituto Nacional de Investigaciones Agrarias, Madrid, Spain.

e Isolate supplied by Dr. P. García-Benavides, Centro Regional de Diagnostico, Junta de Castilla y León, Aldearrubia, Salamanca, Spain.

f Isolate supplied by Dr. Enrique Monte, Universidad de Salamanca, Salamanca, Spain.





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