1 Collection of the Institute of Cytology and Genetics (Novosibirsk, Russia).
2 Collection of the Vavilov Research Institute for Plant Industry (St. Petersburg, Russia)
3 Collection of the Leibniz Institute of Plant Genetics and Crop Plant Research (Gatersleben, Germany)
4 Collection of the Weizmann Institute of Science (Rehovot, Israel).
Table S2. Triticeaeintergenera lines and their parents employed and the outcome of PCR analysis for the presence of the rye Chi gene as marker of rye genome/chromosome 5R. * - chromosome substitution lines (Adonina et al. 2011), ** - chromosome addition lines, *** - ditelosomic chromosome addition lines (Driscoll and Sears, 1971).
Fig. S1. Multiple alignment of the Chi nucleotide sequences in different rye cultivars. Primers are indicated with arrows.
Fig. S2. PCR assay of a part of the mapping population P87/P105 (lines 1-13) and parental lines P87 (line 14) and P105 (line 15) with primer pair Chi_1F/2R (Table 1) separated in 5% HR agarose gel.
Fig. S3. Multiple alignment of the deduced polypeptide sequences encoded by Сhi in different rye cultivars. Residues associated with the substrate binding cleft are shown in green, those with the active site hydrogen bond network in pink, and other conserved residues in blue (following Jez et al. 2000).
Fig. S4. The 3D structure of CHI in Medicago sativa L. (Jez et al. 2000) and in rye cultivars (determined in the current study). The open-faced β-sandwich fold structure was revealed in M. sativa by crystallography (Jez et al. 2000).