Supplementary Figures: Supplementary Fi




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Supplementary Figures:



Supplementary Fig. 1 The secondary structure of the Δ6 desaturase.

Note: The solid line frames were five helical transmembrane regions of Δ6 desaturase based on prediction PredictProtein





Supplementary Fig. 2 Phylogenetic relationships among Δ6 fatty acid desaturase gene from various organisms.

Note: 1. ▲ indicated the submission number of Δ6 fatty acid desaturase gene which cloned from cDNA of Mortierella alpina in this paper. 2. AF465281, Mortierella alpina ATCC 16266; AF307940, Mortierella alpina; AF465282, Mortierella isabellina M6-22; AF306634, Mortierella isabellina; AB020032, Mortierella alpina 1S-4; EF413024, Mortierella alpina W15; AB070555, Mortierella alpina; AF419296, Pythium irregulare; AY630575, Glossomastix chrysoplasta; AY817155, Thalassiosira pseudonana; EF494667, Phaeodactylum tricornutum; AY082393, Phaeodactylum tricornutum UTEX 646; DQ177498, Cunninghamella echinulata; DQ432036, Thamnidium elegans; AY795076, Rhizopus stolonifer R31.6; AY583463, Marchantia polymorpha; AJ250734, Ceratodon purpureus wt3; AJ222980, Physcomitrella patens Hedw.B.S.G.; AJ250735, Ceratodon purpureus wt3; AF031477, Caenorhabditis elegans; AF290983, Mucor rouxii ATCC 24905; AF296076, Mucor rouxii ATCC 24905; AB090360, Mucor circinelloides HUT1121; EF569210, Rhizomucor pusillus; AY234125, Primula farinosa; AY234127, Primula vialii; AF536525, Anemone leveillei; AY169402, Camellia sinensis; U79010, Borago officinalis; DQ067621, Paramecium biaurelia; AY952780, Echium plantagineum; AY055117, Echium gentianoides; AY055118, Echium pitardii var. pitardii; AY746357, Ostreococcus tauri; AF126798, Mus musculus; NM_031344, Rattus norvegicus; AF084559, Homo sapiens; XM_421053, Gallus gallus; AF134404, Homo sapiens(human); AF309557, Cyprinus carpio; AF309556, Danio rerio; AF301910, Oncorhynchus mykiss; AY458652, Salmo salar; AB070444, Oncorhynchus masou; DQ054840, Gadus morhua; AB069727, Oreochromis niloticus; AY055749, Sparus aurata; AY546094, Scophthalmus maximus; L11421, Synechocystis sp.; X87094, Synechocystis platensis Italy; XM_001563387, Leishmania braziliensis MHOM/BR/75/M2904; XM_001687682, Leishmania friedlin; XM_001464284, Leishmania infantum JPCM5; NM_119243, Arabidopsis thaliana; AY772187, Olea europaea subsp. europaea; AB007640, Chlamydomonas reinhardtii; AB031546, Chlamydomonas sp. W80





Supplementary Fig. 3 Phylogenetic relationships among Δ6 fatty acid desaturase from various organisms.

Note: AF465281, Mortierella alpina ATCC 16266; AF307940, Mortierella alpina; AF465282, Mortierella isabellina M6-22; AF306634, Mortierella isabellina; AB020032, Mortierella alpina 1S-4; EF413024, Mortierella alpina W15; AB070555, Mortierella alpina; AF419296, Pythium irregulare; AY630575, Glossomastix chrysoplasta; AY817155, Thalassiosira pseudonana; EF494667, Phaeodactylum tricornutum; AY082393, Phaeodactylum tricornutum UTEX 646; DQ177498, Cunninghamella echinulata; DQ432036, Thamnidium elegans; AY795076, Rhizopus stolonifer R31.6; AY583463, Marchantia polymorpha; AJ250734, Ceratodon purpureus wt3; AJ222980, Physcomitrella patens Hedw.B.S.G.; AJ250735, Ceratodon purpureus wt3; AF031477, Caenorhabditis elegans; AF290983, Mucor rouxii ATCC 24905; AF296076, Mucor rouxii ATCC 24905; AB090360, Mucor circinelloides HUT1121; EF569210, Rhizomucor pusillus; AY234125, Primula farinose; AY234127, Primula vialii; AF536525, Anemone leveillei; AY169402, Camellia sinensis; U79010, Borago officinalis; DQ067621, Paramecium biaurelia; AY952780, Echium plantagineum; AY055117, Echium gentianoides; AY055118, Echium pitardii var. pitardii; AY746357, Ostreococcus tauri; AF126798, Mus musculus; NM_031344, Rattus norvegicus; AF084559, Homo sapiens; XM_421053, Gallus gallus; AF134404, Homo sapiens; AF309557, Cyprinus carpio; AF309556, Danio rerio; AF301910, Oncorhynchus mykiss; AY458652, Salmo salar; AB070444, Oncorhynchus masou; DQ054840, Gadus morhua; AB069727, Oreochromis niloticus; AY055749, Sparus aurata; AY546094, Scophthalmus maximus; L11421, Synechocystis sp.; X87094, Synechocystis platensis Italy; XM_001563387, Leishmania braziliensis MHOM/BR/75/M2904; XM_001687682, Leishmania Friedlin; XM_001464284, Leishmania infantum JPCM5; NM_119243, Arabidopsis thaliana; AY772187, Olea europaea; AB007640, Chlamydomonas reinhardtii; AB031546, Chlamydomonas sp. W80





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