Species Collection Identification




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Table S1. Collecting sites and average of DNA content in all Rivulidae individuals analyzed by flow cytometry.


Species

Collection Identification

Collecting site

Locality reference in the map

Average of DNA content/individual (2C)

Austrolebias charrua (Costa and Cheffe, 2001)

GP2550

La Coronilla pond, Rocha Department, Uruguay

6

6.14 pg*

Austrolebias charrua

GP2325

Route 9, km 272 (pond 6), Rocha Department, Uruguay 

6

5.68 pg

Austrolebias charrua

GP2387

Route 9, km 272 (pond 6), Rocha Department, Uruguay 

6

6.41 pg

Austrolebias charrua

GP2388

Route 9, km 272 (pond 6), Rocha Department, Uruguay

6

6.29 pg

Austrolebias luteoflammulatus (Vaz-Ferreira, Sierra and Paulete, 1964)

GP2390

Route 9, Probides, Rocha Department, Uruguay

13

5.76 pg*

Austrolebias luteoflammulatus

GP2391

Route 9, Probides, Rocha Department, Uruguay

13

5.76 pg

Austrolebias luteoflammulatus

GP2392

Route 9, Probides, Rocha Department, Uruguay

13

5.60 pg

Austrolebias luteoflammulatus

GP2547

Route 9, Probides, Rocha Department, Uruguay

13

6.60 pg*

Austrolebias luteoflammulatus

GP2549

Route 9, Probides, Rocha Department, Uruguay

13

6.22 pg

Austrolebias luteoflammulatus

GP3019

Route 9, km 248,  Rocha Department, Uruguay

13

6.65 pg

Austrolebias luteoflammulatus

GP3020

Route 9, km 248,  Rocha Department, Uruguay

13

6.33 pg

Austrolebias luteoflammulatus

GP3058

Route 9, km 248,  Rocha Department, Uruguay

13

6.43 pg

Austrolebias reicherti (Loureiro and García, 2005)

GP2539

Camino al Arrozal, Treinta y tres Department, Uruguay

24

6.39 pg

Austrolebias reicherti

GP2540

Camino al Arrozal, Treinta y tres Department, Uruguay

24

6.42 pg

Austrolebias reicherti

GP2992

Camino al Arrozal, Treinta y tres Department, Uruguay

24

6.42 pg

Austrolebias reicherti

GP2993

Camino al Arrozal, Treinta y tres Department, Uruguay

24

6.35 pg

Austrolebias prognathus (Amato, 1986)

GP2378

Route 8, next to Jose Pedro Varela Town, Lavalleja Department, Uruguay

22

6.84 pg

Austrolebias prognathus

GP2379

Route 8, next to Jose Pedro Varela Town, Lavalleja Department, Uruguay

22

7.10 pg

Austrolebias elongatus (Steindachner, 1881)

GP2389

Villa Soriano Town, Soriano Department, Uruguay

10

6.05 pg

Austrolebias elongatus

GP2545

Villa Soriano Town, Soriano Department, Uruguay

10

6.57 pg*

Austrolebias elongatus

GP3009

Villa Soriano Town, Soriano Department, Uruguay

10

6.01 pg

Austrolebias elongatus

GP3010

Villa Soriano Town, Soriano Department, Uruguay

10

5.74 pg

Austrolebias elongatus

GP3011

Villa Soriano Town, Soriano Department, Uruguay

10

5.73 pg

Austrolebias quirogai (Loureiro, Duarte and Zarucki, 2011)

GP2536

 Cerro de las cuentas Town, Cerro Largo Department, Uruguay

23

6.28 pg

Austrolebias quirogai

GP2537

 Cerro de las cuentas Town, Cerro Largo Department, Uruguay

23

6.23 pg

Austrolebias quirogai

GP2538

 Cerro de las cuentas Town, Cerro Largo Department, Uruguay

23

6.42 pg

Austrolebias vazferreirai (Berkenkamp, Etzel, Reichert and Salvia, 1994)

GP2385

F1. Parentals from Fraile Muerto, Cerro Largo Department, Uruguay

26

5.78 pg

Austrolebias vazferreirai

GP2386

F1. Parentals from Fraile Muerto, Cerro Largo Department, Uruguay

26

5.70 pg*

Austrolebias cheradophilus (Vaz-Ferreira et al., 1964)

GP2380

Contact Zone 6, Treinta y Tres Department, Uruguay

7

5.51 pg

Austrolebias cheradophilus

GP3013

Route 9, km 248,  Rocha Department, Uruguay

7

6.42 pg

Austrolebias cheradophilus

GP3014

Route 9, km 248,  Rocha Department, Uruguay

7

6.09 pg

Austrolebias nigripinnis (Regan, 1912)

GP2398

F1. Parental from Route 21, Carmelo City, Colonia Department, Uruguay

17

5.31 pg*

Austrolebias nigripinnis

GP2399

F1. Parental from Route 21, Carmelo City, Colonia Department, Uruguay

17

5.94 pg*

Austrolebias wolterstorffi (Ahl, 1924)

GP2381

Next to Vergara City, Treinta y Tres Depatment, Uruguay.

29

5.65 pg*

Austrolebias wolterstorffi

GP2382

Next to Vergara City, Treinta y Tres Depatment, Uruguay.

29

5.69 pg

Austrolebias wolterstorffi

GP2383

Next to Vergara City, Treinta y Tres Depatment, Uruguay.

29

5.48 pg

Austrolebias wolterstorffi

GP2994

F1. Parentals from La Coronilla pond, Rocha Deparment, Uruguay 

29

5.52 pg

Austrolebias wolterstorffi

GP3012

Route 14, km 506, Rocha Deparment, Uruguay 

29

5.39 pg

Austrolebias viarius (Vaz-Ferreira, Sierra de Soriano and Scaglia de Paulete 1964)

GP2995

Route 9, km 248,  Rocha Department, Uruguay

28

6.11 pg

Austrolebias viarius

GP2996

Route 9, km 248,  Rocha Department, Uruguay

28

5.78 pg

Austrolebias viarius

GP2997

Route 9, km 248,  Rocha Department, Uruguay

28

6.12 pg

Austrolebias viarius

GP2998

Route 9, km 248,  Rocha Department, Uruguay

28

5.77 pg

Austrolebias alexandri (Castello and Lopez, 1974)

GP2999

F1. Parentals from Vizcaino Stream, Rio Negro Department, Uruguay

3

5.06 pg

Austrolebias alexandri

GP3000

F1. Parentals from Vizcaino Stream, Rio Negro Department, Uruguay

3

5.36 pg

Austrolebias alexandri

GP3001

F1. Parentals from Vizcaino Stream, Rio Negro Department, Uruguay

3

5.27 pg

Austrolebias cinereus (Amato, 1986)

GP2541-2542

Route 21, Carmelo City, Colonia Department, Uruguay

8

5.61 pg

Austrolebias melanoorus (Amato, 1986)

GP2544

Next to Tacuarembó River, Tacuarembó Department, Uruguay

15

5.61 pg *

Austrolebias duraznensis (García, Scvortzoff and Hernández, 1995)

GP2326

Next to Mercedes, Soriano Departement, Uruguay 

9

5.88 pg

Austrolebias duraznensis

GP2396

Next to Mercedes, Soriano Departement, Uruguay 

9

5.92 pg*

Austrolebias duraznensis

GP2397

Next to Mercedes, Soriano Departement, Uruguay 

9

8.74 pg

Austrolebias duraznensis

GP2546

Next to Mercedes, Soriano Departement, Uruguay 

9

6.02 pg

Austrolebias duraznensis

GP2548

Next to Mercedes, Soriano Departement, Uruguay 

9

5.63 pg*

Austrolebias duraznensis

GP3061

Next to Mercedes, Soriano Departement, Uruguay 

9

6.10 pg

Austrolebias gymnoventris (Amato, 1986)

GP2393

 Salamanca Caves, Maldonado Department, Uruguay

11

5.26 pg*

Austrolebias gymnoventris

GP2394

 Salamanca Caves, Maldonado Department, Uruguay

11

5.54 pg*

Austrolebias gymnoventris

GP2395

 Salamanca Caves, Maldonado Department, Uruguay

11

5.60 pg*

Austrolebias gymnoventris

GP3015

 F1. Parentals from Salamanca Caves, Maldonado Department, Uruguay

11

5.62 pg

Austrolebias gymnoventris

GP3016

 F1. Parentals from Salamanca Caves, Maldonado Department, Uruguay

11

5.28 pg

Austrolebias gymnoventris

GP3017

 F1. Parentals from Salamanca Caves, Maldonado Department, Uruguay

11

5.51 pg

Cynolebias melanotaenia (Regan, 1912)

R443

Route 9, km 272 (pond 6), Rocha Department, Uruguay 

C

2.76 pg

Cynolebias melanotaenia

R447

Route 14, km 506, Rocha Deparment, Uruguay 

C

2.67 pg


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