Progress in research on breeding for resistance to broomrape José M. Fernández-Martínez, Leonardo Velasco, Begoña Pérez-Vich




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Progress in research on breeding for resistance to broomrape
José M. Fernández-Martínez, Leonardo Velasco, Begoña Pérez-Vich

Instituto de Agricultura Sostenible (CSIC). Alameda del Obispo s/n. E-14004 Córdoba, Spain



e-mails: jfernandezm@ias.csic.es; lvelasco@ias.csic.es; bperez@ias.csic.es

ABSTRACT


  • Sunflower broomrape (Orobanche cumana Wallr.) is a parasitic angiosperm regarded as one of the most serious production constraints in many sunflower-producing countries. Historically, sunflower breeders have been successful in developing cultivars resistant to this parasite but the introduction of new resistance sources has been frequently followed by the appearance of new pathogenic races overcoming the resistance. The present review sets out to examine the principal achievements in this field that took place during the last decades focusing in the most recent advances in research.

  • In recent years, several research groups have made relevant contributions on breeding for resistance and development of control strategies. Following the identification and characterization of new virulent races, new sources and mechanisms of resistance have been developed and characterized at the genetic and molecular level. There have been also important contributions to the knowledge of the biology and genetics of the parasite that will contribute to understand the dynamics of broomrape populations and race evolution. Important advances have also been made in the use of imidazolinone (IMI)-resistant hybrids as an alternative method of control.

  • Most of the studies on sunflower breeding for resistance to O. cumana have been concentrated so far in the host plant but, in spite of recent advances, little in known in important aspects of the parasite side such as breeding system and genetics of virulence. An unsolved problem is the existing uncertainty in the designation of races and resistance genes identified in different countries. There is a need for international cooperation in this area using common differentials and molecular tools. Emphasis is given to the need for a multidisciplinary approach ranging from studying the mechanisms underlying resistance in the host plant to the reproductive biology and genetics of traits related to parasitism in O. cumana. The combination of long term strategies involving the complete host-parasite system and the integration of classical and molecular approaches is expected to contribute to the development of more durable sources of resistance and to improve sunflower performance in areas infested by broomrape.


Key words: Broomrape races – genetic resistance – herbicide tolerance – HelianthusOrobanche – sunflower

INTRODUCTION
Sunflower broomrape (Orobanche cumana Wallr.) is a holoparasitic plant that currently represents one of the most serious production constraints in many sunflower-producing countries around the world, especially in Central and Eastern Europe, Spain, Turkey, Israel, Iran, Kazakhstan, and China (Škorić et al., 2010). Broomrape attacks are frequently severe and yield losses can reach up to 50% (Domínguez, 1996a). Historically, sunflower breeders have been successful in developing broomrape resistant cultivars but breeding programs are often based on a reduced number of dominant genes and resistance breakdown caused by the appearance of new virulent races that overcome all known resistance genes has frequently occurred in the last decades (Fernández-Martínez et al., 2008). This situation has forced sunflower breeders to continuously search for new sources of resistance and/or using alternative methods of control such as the use of imidazolinone (IMI)-resistant hybrids. The aim of this review is to discuss recent advances in research in the development and genetic characterization of new sources of resistance to new virulent races, as well as in the biology and genetics of the parasite and in the development of IMI- resistant hybrids.
DISCUSSION
Reproductive biology of the parasite

Studies on sunflower resistance to O. cumana have been mainly concentrated on understanding the mechanisms underlying resistance in the host plant, but little in known on the parasite side. There is scarce information about the reproductive biology of the parasite, the genetic structure of the populations and the mode of inheritance of traits related to parasitism. Recently, a methodological study was conducted to test the feasibility of bagging O. cumana plants to produce seed from self-fertilization and controlled hybridization. The results revealed that O. cumana is self-compatible and tolerates seed production under isolation, both under self-fertilization as well as in controlled hybridization, although the bag type used for isolation clearly determined seed production. Best results were obtained for plants isolated with micro-perforated transparent plastic bags, which produced seeds that did not differ in germination and infectivity capacity from these produced in unbagged control plants (Rodríguez-Ojeda et al., 2010). Using these techniques, an unpigmented mutant lacking anthocyanin was isolated and the inheritance of the trait was studied in crosses with normal pigmented plants followed by evaluation of the F1, F2 and F3 plant generations. The results revealed that the unpigmented trait was controlled by partially recessive alleles at a single locus, with the F1 hybrid being phenotypically distinguishable from both pigmented and unpigmented parents (Rodríguez-Ojeda et al., 2011). The feasibility of identifying phenotypically F1 plants from unpigmented mutant plants has encouraged the use of the mutant as a phenotypic marker for estimating the rate of natural cross-fertilization in O. cumana, which was found to be between 14.8 to 40.0% in two separate experiments (Rodríguez-Ojeda et al., unpublished results). These results partially contradict the hypothesis of Gagne et al. (1998) who, based on the low intra-population variability detected in O. cumana populations, suggested that O. cumana is a self-pollinated species.


Dynamics of broomrape populations and race evolution

In the wild, O. cumana mainly parasitizes Artemisia spp. but, shortly after the expansion of sunflower cultivation in Russia at the end of the 19th century, O. cumana started parasitizing this crop (Vrânceanu et al., 1986). Genetic resistance was initially introduced into susceptible sunflower mainly from the wild species H. tuberosus (Škorić et al., 2010). However, new pathogenic races overcoming resistant cultivars appeared. In a classical study on the host-parasite system sunflower-Orobanche, Vrânceanu et al. (1980) indicated that it followed the gene-for-gene model and identified five pathogenic races in Romania, named A through E, with a set of sunflower differentials carrying the dominant resistance genes Or1 through Or5. A new virulent race named F overcoming resistance gene Or5 was identified in Spain in the mid 1990s (Alonso et al., 1996). Races overcoming all the known resistance genes to races A through E were also identified in other countries such as Romania, Turkey, Ukraine, Bulgaria and Russia. They have been named as F, G, and H (Fernández-Martínez et al., 2008; Păcureanu-Joita et al., 2009; Škorić et al., 2010). However, no consistent comparative studies have been conducted on the virulence of broomrape populations from different countries overcoming Or5 resistance. Therefore, the current racial situation of broomrape in the main infested areas remains unclear, i.e. there is no information on whether races under the same classification reported in different countries are the same or they differ for virulence. International cooperation to clarify the current racial status of sunflower broomrape using a collection of different races, common differential sets and molecular tools is becoming a top priority in sunflower research, taking particularly into account that broomrape is spreading into new areas such as some regions in Spain (Fernández-Escobar et al., 2009) and France (Jouffret and Lecomte, 2010).

In order to understand race evolution, a basic knowledge of the genetic structure and dynamics of O. cumana populations is required. Molecular studies on genetic diversity of sunflower broomrape concluded that there is low intra-population and large inter-population genetic variation with little gene exchange between regions (Gagne et al., 1998). However, the study of 38 old seed accessions of O. cumana in Spain provided evidence of heterogeneous populations with high virulence components (race F) in old populations collected prior the identification of this race, suggesting selection pressure of resistance genes in these heterogeneous populations as a major evolution force building up new virulent races such as race F (Molinero-Ruiz et al., 2008). The high virulent components could be the result of recent mutation but, given the ease at which minuscule Orobanche seeds can be transferred from one place to another, they could have derived from seed introductions from other areas as reported in Spain (Fernández-Escobar et al., 2009). Genetic recombination may also play an important role in race evolution in O. cumana on account of the relatively high rate of outcrossing observed in this species, as mentioned in the previous section.
Sources of resistance

Unlike other host plant-Orobanche systems, sources of resistance to O. cumana in sunflower have been in most cases based on vertical resistance mechanisms, controlled by single dominant genes. This has led to a rapid breakdown of the resistance and subsequently to a continuous need for new resistance sources. In the early breeding sunflower work in the former USSR and Romania, sources of resistance to the first races were identified within landraces and also in wild species, mainly H. tuberosus (Vrânceanu et al., 1980; Škorić et al., 2010). As new broomrape races were appearing, sources of resistance in the germplasm of cultivated sunflower became increasingly scarce, though valuable germplasm resistant to the latest races has still been identified, including germplasm with horizontal instead of vertical resistance (Fernández-Martínez et al., 2008; Păcureanu-Joita et al., 2009; Škorić et al., 2010). In contrast, a high level of resistance to the latest races has been found in wild Helianthus species. For example, an extensive evaluation of 54 wild accessions for resistance to several virulent races, included Spanish race F identified a high level of resistance in 29 perennial and four annual Helianthus species (Fernández-Martínez et al., 2000). The outstanding work carried out in Bulgaria during decades has produced a number of resistance sources to races from A to G from interspecific hybridization with 32 wild species (Christov et al., 2010). Recently, resistance to a race named as G has been transferred from H. debilis (Velasco et al., 2012). Transferring resistance genes from cultivated germplasm and wild annual species into elite germplasm can be accomplished rather easily by conventional crossing and backcrossing. Conversely, introgression of resistance from wild perennial species is generally more difficult due to problems associated with early hybrid embryo abortion and sterility in the F1 and BC1 generations and may require special techniques such as embryo rescue and chromosome doubling of the F1 (Jan and Fernández-Martínez, 2002).


Characterization of resistance

Several comprehensive reviews on inheritance of sunflower broomrape resistance have been recently published (e.g. Fernández-Martínez et al., 2008; Škorić et al., 2010). Most genetic studies on different resistance sources to races A through E concluded that a major dominant gene conferred resistance to the latest race and to the previous ones (Vrânceanu et al., 1980; Sukno et al., 1999), even though other genetic mechanisms such as two dominant genes were also reported (Domínguez, 1996b). In contrast to races A though E, initial genetic studies on resistance to race F concluded the presence of recessive alleles at two independent loci named Or6 and Or7 (Rodríguez-Ojeda et al., 2001; Akhtouch et al., 2002). Contrarily, Pérez-Vich et al. (2002) found that resistance to race F in sunflower line J1 derived from wild species was controlled by dominant alleles at a single gene designated Or6. Further research revealed that dominance depended upon the susceptible parental line used for the cross, as in some cases F1 hybrids segregated for resistance or were susceptible, which was attributed to the presence of a minor or modifying gene in some of the susceptible lines (Velasco et al., 2006). More recent studies on the inheritance of resistance to the latest races in several countries have concluded the presence of one or two dominant genes (Škorić et al., 2010; Velasco et al., 2012). Similarly to the problem of broomrape race nomenclature discussed above, no comparative genetic studies have been conducted on sunflower resistant germplasm developed by different authors, for which the same gene nomenclature Or6 and Or7 has been used.

Molecular studies aimed at mapping and tagging genes conferring resistance to races E and F have been carried out. These studies revealed that the Or5 gene conferring resistance to race E is located on a telomeric region of linkage group (LG) 3 of the sunflower genetic map (Lu et al., 2000; Tang et al., 2003; Pérez-Vich et al., 2004). The nature of this dominant gene is unknown, though it has been hypothesized that it might be a cluster of recognition-dependent resistance genes encoding proteins characterized by the presence of leucine-rich repeat (LRR) motifs and a nucleotide binding site (NBS) N-terminal to the LRR domain (Lu et al., 2000). This is supported by the mapping of three NBS-LRR loci to the upper segment of LG 3 closely linked to Or5, two of them derived from H. tuberosus (Radwan et al., 2008), which has been extensively used as a source of broomrape resistance genes (Fernández-Martínez et al., 2008). Quantitative trait loci (QTL) analyses showed that, in addition to the major gene Or5, resistance to race E has also a quantitative component determined by four QTLs with minor effects, in some cases non-race specific and mainly associated with the number of broomrape stalks per plant (Pérez-Vich et al., 2004). The existence of this polygenic component in race E resistance is supported by histological observations and gene expression studies that demonstrated that several mechanisms such as reduced stimulation of broomrape seed germination or rapid necrosis at early stages of parasite development are involved in resistance to this broomrape race (Labrouse et al., 2001; Pérez de Luque et al., 2009). In contrast to race E, molecular studies concluded that recessive resistance to race F in sunflower line P-96 was determined by six QTL, some of which were non-race specific and stable over environments (Pérez-Vich et al., 2004). Several resistance mechanisms to broomrape race F such as physical barriers involving cell wall suberization and protein cross-linking as well as chemical response by producing and secreting toxic compounds of a phenolic nature have been identified (Echevarría-Zomeño et al., 2006). In order to improve the durability of resistance, breeding strategies such as pyramiding major genes or combining vertical and horizontal resistance mechanisms must be implemented. In line with this, Hassan et al. (2011) reported the production of a strongly resistant hybrid from the combination of lines carrying two different resistant mechanisms, one of them consisting in the failure of inducing Orobanche seed germination (called system 1) and the other one consisting in the necrosis of the parasite structures at an early stage of development (called system 2).
Use of herbicide-tolerant cultivars

The rapid evolution of broomrape populations, which have shown strong capacity to overcome new resistance sources and to spread to new areas, induced sunflower breeders to consider alternative methods of broomrape control. The imidazolinone (IMI) family of herbicides controls weeds by inhibiting a key enzyme in the branched chain amino acid biosynthetic pathway, acetohydroxyacid synthase (Tan et al., 2005). In 1996, a wild sunflower population of H. annuus, highly tolerant to the IMI herbicide imazethapyr, was found in a soybean field in Kansas, US (Al-Khatib et al., 1998). Seed specimens of this material were used by the USDA Agricultural Research Service at Fargo, ND to introgress genes underlying the herbicide tolerance into cultivated sunflower. Genetic stocks and breeding lines with IMI herbicide resistance were developed and released for the development of commercial resistant hybrids (Al-Khatib and Miller, 2000). The commercial IMI tolerance trait Imisun that arose from that introgression work had a great impact in sunflower breeding, not only because of the extensive list of weeds controlled in post-emergence by IMI herbicides, but also because they proved to be highly efficient in controlling sunflower broomrape (Alonso et al., 1998). Consequently, IMI-tolerant sunflowers, currently commercialized under the Clearfield trademark, have gained a market share very quickly once the trait has been incorporated into performing hybrids by most of the sunflower seed companies. The inheritance of Imisun was found to be controlled by one gene Imr1, also known as Ahasl1-1, exhibiting partial dominance, which is affected by a second gene Imr2 with modifier or enhancer effect in some genetic backgrounds (Bruniard and Miller, 2001). To produce Imisun sunflower hybrids that express commercial tolerance levels to IMI herbicides, both genes need to be in homozygous state. A new IMI-resistant trait initially designated CLHA-plus and later CLPlus was developed by mutagenesis and selection with imazapyr (Sala et al., 2008). Molecular and genetic studies concluded that CLPlus is controlled by the expression of the partially dominant allele Ahasl1-3 that is different from Ahasl1-1. Both of them are allelic variants of locus Ahasl1, one of three genes coding for the AHAS catalytic subunits in sunflower (Sala et al., 2008). Lines and hybrids carrying the CLPlus mutation have better tolerance to IMI herbicides and commercial tolerance levels can be achieved incorporating CLPlus in only one parent of a Clearfield hybrid, thus allowing more rapid development of the hybrid. The allele Ahasl1-3 has been found to be dominant over Ahasl1-1 (Sala et al., 2012). New sources of resistance to IMI herbicides have been recently identified in populations of wild H. annuus and H. argophylus. (Christov et al., 2010).


CONCLUSIONS AND FUTURE PROSPECTS

Sunflower broomrape continues to be one of the most serious production constraints in many sunflower-producing countries around the world after more than a century. Race-specific dominant genes have been considered to be the ideal source of resistance for single-cross hybrid breeding by seed companies, because they only need to be incorporated into one of the parents, but its extensive use has contributed to the rapid evolution of new virulent races. This requires a continuous search for new resistance sources. Wild Helianthus species constitute the major source of resistance genes conferring resistance to new virulent races, but cultivated germplasms have provided also valuable sources of resistance. Most of the resistant sources have been found to be controlled by major genes (vertical resistance), although quantitative (horizontal) resistance has also been reported. With this situation, a set of integrated strategies including the use of herbicide-tolerant cultivars to control broomrape and the development of more durable genetic resistance is needed. Novel approaches to improve durability of broomrape resistance include the combination of vertical and horizontal resistance mechanisms assisted by the use of molecular markers. Reproducible molecular markers for accurately identifying O. cumana races need also to be developed. The combination of such strategies is expected to contribute to improve sunflower performance in areas infested by broomrape.


ACKNOWLEDGEMENTS

The authors wish to thank the organizers of the 18th International Sunflower Conference for their invitation to write this review.


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