Production of a monoclonal antibody in Nicotiana tabacum in vitro culture




Дата канвертавання24.04.2016
Памер3.97 Kb.
Production of a monoclonal antibody in Nicotiana tabacum in vitro culture
A. López1, J. Parsons 1, C. Martínez1, S. Petrucelli 2, T Fernández 3, A.M. Giulietti 1, M.A. Álvarez 1

1Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, UBA,2CIDCA, Facultad de Ciencias Exactas, UNLP, La Plata,3Cátedra de Inmunología, Facultad de Farmacia y Bioquímica, UBA- Argentina. e-mail: malvarez@ffyb.uba.ar
The potential of plants as “factories” for the production of recombinant antibodies (plantibodies) opens up new opportunities, not only for the medical science, but also for applied and fundamental agronomic research. The approach to plantibodies in in vitro cultures is promising for cost-efficiency and biosafety, especially for therapeutical applications. The antibody 14D9 is a mouse IgG1 that catalyzes the hydrolysis of enol- ethers in an enantiospecific way with a high specific activity. Methods that use catalytic antibodies may be pivotal both in providing a tool to generate tailor-made catalysts and in creating models of enzymes involved in a number of metabolic diseases. 14D9 was successfully expressed in N. tabacum plants with and without the retention sequence EKDEL.

Our goal is to demonstrate the ability of N. tabacum in vitro cultures (calli and suspension cultures) to express 14D9. Different cell lines were obtained whose 14D9 expression levels were correlated to those of the parental plant. The highest values obtained were between 1.00-1.60 mg % of the total soluble protein. We have also successfully established hairy roots from the transgenic plants. Further studies are being conducted in order to establish the influence of several cultural conditions and of differentiation on 14D9 antibody expression level.


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