„Molecular Biology” course reviews basic topics within mammalian genetics, cell biology and molecular biology, and looks for possible applications of these topics in medicine. Students will refresh and update their knowledge on advances in gene expression, from DNA to RNA and to the mature protein products. The course includes an experimental part of either genetics, cell biology or molecular biology of bacterial or mammalian cells. During experimental part students can solve some problems, for example: screening some useful genes for anti-cancer therapy or isolate the DNA.
dr n. med. Anna Bajek, dr n. med. Celestyna Mila-Kierzenkowska,
Anna Bajek: firstname.lastname@example.org, Celestyna Mila-Kierzenkowska: email@example.com
Name of the unit: Chair of Medical Biology
Head of the Unit: dr hab. Alina Woźniak, prof. UMK
Faculty of Medicine, Medical Program, first year
Course coordinator: dr hab. Alina Woźniak, prof. UMK
Form of the classes: lectures, tutorials
Form of crediting/assessment: exam, 3 ECTS points
Number of hours: lectures 20 hours, tutorials 10 hours
The aim of teaching:
“Molecular Biology” program is realized in the first semester. The general objectives of the module are to teach students basic genetics and molecular biology, and to provide insight into the principles and applications of molecular genetics techniques. Another core objective is to provide knowledge that enables students to reflect on when and how molecular genetics techniques can and should be applied in screening, diagnosis and treatment.
The course contents:
Cell structure and cell division. Cell life cycle and its disorders. Stem cells. Cell differentiation and aging process. Types of cell death. The structure and functions of protein. Posttranslational and functional modifications of protein. The structure of DNA. Chemical, physical, spectroscopic and thermal properties of DNA. Methods of DNA isolation. Chromatin structure. Sex chromatin. Structure of prokaryotic and eukaryotic chromosomes. The complexity and function of the genome. Mitochondrial genome. Molecular methods for the study of the human genome: genetic engineering as the basis for molecular diagnostics, molecular methods used in the diagnosis of malformations and genetic diseases, molecular marker systems. DNA replication in Procaryota and Eucaryota. DNA damage, repair and recombination. The types of mutations, and examples of their record rules. Factors that increase the genetic diversity of the population. The mechanisms of aging, theories of aging. Types and functions of RNA. The genetic code. Mechanism of transcription in Procaryote and Eucaryote. Maturation of RNA and RNP particles. The mechanism of protein synthesis. Control of translation and posttranslational events. Regulation of gene expression. Molecular basis of cancer: activation of protooncogenes and inactivation of tumor suppressor genes.
1. Genomes. Brown TA. Oxford: Wiley-Liss, the latest edition.
2. Molecular Biology and Biotechnology. 5th edition. Walker JM, Rapley R. RSC Publishing, 2009.
3. Essential Medical Genetics. Connor M, Ferguson-Smith MA. Blackwell Science Ltd, the latest edition.
4. Genetics. Freidman JM, Dill FJ, Hayden MR, McGillivray BC. Williams&Wilkins, the latest edition.
Rules and regulations
I. Form and criteria of the course completion:
The prerequisite is completion of practical classes and to obtain at least 60% of points from the final exam. Gain a minimum of 60 points during classes gives the possibility of exemption from examination, and obtaining 54-59 points allows the student to take the exam in ‘time zero’. The exam is in the form of the test and covers the issues discussed in the lectures and classes and in the given literature. A student who fails the exam, according to the regulations of study, has a single chance to improve the grade.
II. Form and criteria of classes completion:
The prerequisite is to obtain min 40 points. The maximum number of points, possible to obtain during classes, is 67 points. This number includes:
3 tests at the beginning of the classes: 3 x 10 points = max. 30 points
Report from the classes: 3 x 10 points = max. 30 points
Homework: max. 7 points
The tests at the beginning of the each classes will include the current material. The first test will be conducted on the first class. Student is obliged to familiarize with the material contained in the program of the subject and prepare for classes according to given literature. There is only one term of passing these tests, without possibility of improvement.
Reports from the classes should be prepared according to the example obtained during first class and they should be returned at the end of current classes.
Unexcused absence of student on the classes is associated with the lack of opportunities to gain points for the current class. A student who left class and give the excuse is obligated to execute the class after consultation with teacher. Student who has more than two absences during classes will have no chance to complete the course.