Matrine is an alkaloid found in kinds of Sophora plants mainly including Sophora flavescens, Sophora alopecuroides and Sophora subprotrata. It has a wide range of pharmacological actions, including anti-inflammatory, analgesic, antiarrhythmic, antitumour, antifibrotic, anti-diarrhea and immunosuppressive effects. In recent years, matrine has been used in the treatment of chronic liver disease and has a significant effect on the inhibition of liver fibrosis. Salvianolic acid B is one of the water-soluble components from Traditional Chinese Medical Salvia miltiorrhiza. Previous studies have shown that the chemical is effective in improving liver function; alleviating ischemic damage, antioxidation, antihepatotoxity, anticoagulation and antithrombus activity. The newly prepared Matrine salvianolic acid B salt is the synthetic compound by acid-base reaction using Matrine and Salvianolic acid B, which is first reported in this article. Previous studies have shown its protective effect against acute hepatotoxicity by reducing serum AST and ALT levels induced by the treatment of CCl4, Thioacetamide(TTA) and D-Galactosamine (D-GalN) respectively in mice. The present study was to investigate the protective effects of Matrine salvianolic acid B salt on the liver fibrosis induced by CCl4.