| Dear Members and Colleagues
ISTA Seed Health Committee Official Method Review
The Seed Health Method Validation Programme requires that methods approved as Official Methods should be reviewed every 5 years to ensure their continuing effectiveness and suitability. Official Seed Health Testing Methods are found in the International Rules for Seed Testing 2012, Annexe to Chapter 7, Seed Health Testing Methods. There are currently 11 ISTA Official Seed Health Testing Methods that are now due for review (listed in Table 1). This is the third review for Methods 7-001 and 7-002, Methods 7-015 to 7-018 and the first review for Methods 7-022 to 7-024. Table 1 lists the actions taken since the last review as a result of feedback received. The ISTA Seed Health Committee (SHC) would like to receive your feedback on the current Method Sheets as part of the review process.
Table 1. The 11 ISTA Official Seed Health Testing Methods to be reviewed in 2013
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Method No.
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Pathogen
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Host
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Actions taken since last review
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7-001a (Blotter)
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Alternaria dauci
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Daucus carota
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7-001b (Malt agar)
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Alternaria dauci
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Daucus carota
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Streptomycin added to Rules 2013
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7-002 a (Blotter)
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Alternaria radicina
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Daucus carota
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7-002b (Malt agar)
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Alternaria radicina
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Daucus carota
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Streptomycin added to Rules 2013
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7-015
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Neotyphodium spp.
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Festuca and Lolium spp
.
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7-016
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Phomopsis complex
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Glycine max
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7-017
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Alternaria linicola
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Linum usitatissimum
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New method to be voted in June 2013 to align methods 7-007, 7-017 &7-018.
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7-018
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Colletotrichum lini
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Linum usitatissimum
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New method to be voted in June 2013 to align methods 7-007, 7-017 &7-018.
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7-022
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Microdochium nivale
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Triticum spp.
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Not applicable, first review
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7-023
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Pseudomonas savastanoi pv. phaseolicola
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Phaseolus vulgaris
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Not applicable, first review
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7-024
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Pea Early-Browning Virus and Pea Seed-borne Mosaic Virus
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Pisum sativum
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Not applicable, first review
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To help us we would be pleased if you could answer the questions in the accompanying questionnaire and return it to the ISTA Secretariat at the address below by 15 April 2013, or by email to: tcom@ista.ch. The Questionnaire will also be available to non-members through the ISTA website www.seedtest.org.
INTERNATIONAL SEED TESTING ASSOCIATION SEED HEALTH COMMITTEE METHOD REVIEW QUESTIONAIRE: 15 APRIL 2013
1. Name of laboratory:
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Address of laboratory:
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Country:
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1a. Please indicate in the table below which ISTA Official method(s) your laboratory uses?
1b. Are the methods suitable for the purpose they are used for? Please indicate in table below yes or no.
Table for Answers to Questions 1a & 1b (above)
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Method No.
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Pathogen/Host
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Please Tick () method used
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Fit for purpose?
Yes/No
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7-001a (Blotter)
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Alternaria dauci / Daucus carota
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7-001b (Malt agar)
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Alternaria dauci / Daucus carota
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7-002 a (Blotter)
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Alternaria radicina / Daucus carota
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7-002b (Malt agar)
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Alternaria radicina / Daucus carota
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7-015
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Neotyphodium spp. / Festuca and Lolium spp
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7-016
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Phomopsis complex / Glycine max
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7-017
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Alternaria linicola / Linum usitatissimum
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7-018
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Colletotrichum lini / Linum usitatissimum
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7-022
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Microdochium nivale / Triticum spp.
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7-023
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Pseudomonas savastanoi pv. phaseolicola / Phaseolus vulgaris
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7-024
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Pea Early-Browning Virus and Pea Seed-borne Mosaic Virus / Pisum sativum
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1c. If “NO” please specify for each method why you consider the method not to be suitable. (Please use an additional sheet where necessary)
2. Where method is considered fit for purpose do you think further improvement is necessary? Use table.
Table 2
Method No.
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Pathogen/Host
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Key word for desired improvement* and briefly explain
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Priority for improvement**
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7-001a (Blotter)
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Alternaria dauci / Daucus carota
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7-001b (Malt agar)
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Alternaria dauci / Daucus carota
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7-002 a (Blotter)
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Alternaria radicina / Daucus carota
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7-002b (Malt agar)
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Alternaria radicina / Daucus carota
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7-015
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Neotyphodium spp. / Festuca and Lolium spp
.
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7-016
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Phomopsis complex / Glycine max
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7-017
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Alternaria linicola / Linum usitatissimum
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7-018
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Colletotrichum lini / Linum usitatissimum
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7-022
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Microdochium nivale / Triticum spp.
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7-023
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Pseudomonas savastanoi pv. Phaseolicola / Phaseolus vulgaris
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7-024
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Pea Early-Browning Virus and Pea Seed-borne Mosaic Virus / Pisum sativum
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* Key words could be specificity, sensitivity, reproducibility, alternative reagents etc.
** High or low priority
3. Is there a need to respond to a technological change in one or more methods? If yes explain in a few words.
4. Most methods are not validated for use on treated seed. Do you have a need to test treated seeds for these pathogens? If yes please give a brief explanation.
Please note no method modification (except editorial) will be made as a result of this review. A method will either be accepted for a further five years or recommended for withdrawal due to the evidence submitted. Method modifications can only be approved if a full collaborative test has been submitted to the Method Validation Programme and approved by the SHC or depending on the modification when a convincing argument and supporting documentation have been supplied and approved by the SHC.
PLEASE RETURN TO THE ISTA SECRETARIAT (tcom@ista.ch) BY 15 April 2013
YOUR HELP WITH THIS REVIEW IS MUCH APPRECIATED. |