|INCREASING TEMPERATURE HAS INCREASING EFFECT ON PARAMECIUM CAUDATUM
By Pat Tomco
Paramecium cultivated in a controlled setting should increase in population size as the temperature increases. We monitored the growth of paramecium caudatum for five days in the UAF biology lab incubators at four different temperatures. The growth of paramecium was abundant in high temperature environment, while lower temperatures had a slower growth rate. We concluded that higher temperatures cause higher growth rates in paramecium caudatum.
The temperature of the environment should influence the growth characteristics of populations. Paramecium, like any organism requires an adequate amount of heat to survive. When an organism lives in a warmer temperature, less effort will be required for simple life processes, such as photosynthesis, and more importantly, reproduction. Greater availability of heat should lead to a healthier and more populous community of paramecium. In colder temperatures, Cilia on paramecium will also be slowed in lower temperatures, causing slower movement (which is essential for life processes) (author unknown, 2001). This in turn would cause the paramecium to grow more abundantly in higher temperatures.
The purpose of this study was to determine if, and why paramecium grew more abundantly in higher temperatures, compared to lower temperatures. This importance of this relates to the ability of single-celled organisms to reproduce in a warmer environment in that when single-celled organisms such as bacteria enter a host, the environment is going to be a warmer and cause the species to flourish (umich.edu, 1999). We predicted that paramecium would grow more abundantly in high temperatures, due to the ease and facilitation of life processes that heat brings.
We sampled 8 different cultures of paramecium at 4 different temperatures. 5 paramecium were placed into each 40mL tube filled with pasteurized pond water, along with 1mL of paramecium food. Two cultures were placed in each of the four climates (16 degrees C, 24 degrees C, 30 degrees C, and room temperature). On five consecutive days, the total populations within each climate were estimated, and the results were recorded.
We marked off each culture in its individual climate, and proceeded to estimate the population of each culture; 5 different 1mL samples were taken from each culture, counted, then multiplied by 8 to simulate the population at 40mL; The paramecium were then replaced. Total population size was calculated based on these counts.
Clear differences were observed between the paramecium growing in 30 degrees C and the rest of the paramecium grown in this experiment (fig. 1). As we increased the temperature of the environment, the population of paramecium increased. This occurred (more or less) in all of the climates studied.
We concluded that temperature had an effect on the growth of paramecium caudatum grown at the UAF biology lab. In our experiment, two of the eight cultures of paramecium studied were exposed to a climate that was definitely warmer and more desirable (30 degrees C) to an organism of its type. Since there was a sufficient amount of resources and no predators involved, any organism given a similar situation would be expected to flourish (Schwaegerle, 2001). Since the variables that cause erratic growth were restrained, and since both populations did flourish, temperature becomes the variable that causes the growth (or lack thereof) of the paramecium.
In this experiment, we found that the growth of paramecium was not as high (proportionally) for the lower temperatures, as it should be. In other words, we believed that the paramecium at 24 degrees C and at room temperature (very similar temperatures) would have a higher population than it did. We believe this to be a case of trauma As mentioned earlier, when an organism lives in a warmer temperature, less effort will be required for simple life processes. When we returned the sampled paramecium to their environment, there was probably a degree of trauma involved. Since simple life processes were already complicated enough for the organisms in the lower climate, the trauma of being sampled probably had an impact on the lower temperature cultures. The obvious thought here is to not replace the paramecium; however if the paramecium aren't replaced, the results would turn out to have a greater error than if you were to replace them. For instance, if a culture of paramecium after 3 days has 10 individuals, and 6 are sampled, the growth rate of the culture will be drastically reduced.
"Competition," Author unknown, http://www.sprl.umich.edu/GCL/Notes-1999-Fall/competition.html, 1999
"Science - A way of learning about nature," Kent Schwaegerle,
“Creation Evolution Headlines,” Author unknown, Bible Science Association Scientific Journal,” March, 2001 http://creationsafaris.com/crev03.htm