|Evaluation of the antimicrobial activity of Aristolochia longa L. extracts
Merouani N.1, Belhattab R.2, Sahli F.3
1-Department of Vegetal Biology and Ecology, Faculty of Nature and Life Sciences. University Ferhat Abbas –Setif 1– 19000, Setif, Algeria
2- Dept. Biochemistry, Faculty of Nature and Life Sciences. University Ferhat Abbas –Setif 1– 19000, Setif, Algeria
3- Microbiology Laboratory, Faculty of Medicine, University Ferhat Abbas –Setif 1– 19000, Setif, Algeria
Aristolochia longa L. (Aristolochiacea) is a native plant of Algeria used in traditional medicine. Three extracts (methanol, acetone and water) were prepared from stem, leaves, fruits and rhizome and screened for antibacterial activity. The agar diffusion method was assessed to evaluate the efficacy of these extracts against standard strains, Pseudomons aeroginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Bacillus cereus ATCC 10876.
The fruit methanol extract was too efficient against P. aeroginosa , S. aureus and B. cereus with 20mm, 18mm and 15mm inhibition zones respectively. Almost similar results were observed with the acetone extracts from the aerial parts (P.aeroginosa 19mm , S.aureus 14mm, B. cereus 17,5mm) and fruits (P.aeroginosa 17mm ,S. aureus 15mm, B. cereus 17mm) when. The aerial parts aqueous extract was efficient against S. aureus (22mm) and P. aeroginosa (11,6mm),while the effect of the fruit aqueous extract was limited to S. aureus (16,3mm).Rhizome’s extracts effect was weak. Except rhizome methanol and acetone extracts has an impact only on the B. cereus (respectively 12,5 mm and 9mm). This antibacterial activity of these extracts was confirmed by the microdilution method (minimum inhibitory concentrations (MIC)).
No effect was observed when these extracts were tested on the fungi Aspergillus flavus NRRL 391, Aspergillus niger 2CA 936, and Condida albicans ATCC1024.
Keywords: Aristolochia longa L., antibacterial activity, antifungal activity, MIC, extracts