E tg/200/1 original: English date

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		E TG/200/1 ORIGINAL: English DATE: April 9, 2003
INTERNATIONAL UNION FOR THE PROTECTION OF NEW VARIETIES OF PLANTS
GENEVA

BASIL
(Ocimum basilicum L.)

^{^*}

GUIDELINES
FOR THE CONDUCT OF TESTS
FOR DISTINCTNESS, UNIFORMITY AND STABILITY

Alternative Names:^*

Latin	English	French	German	Spanish
Ocimum basilicum L.	Basil	Basilic	Basilikum	Albahaca

ASSOCIATED DOCUMENTS

These guidelines should be read in conjunction with document TG/1/3, “General Introduction to the Examination of Distinctness, Uniformity and Stability and the Development of Harmonized Descriptions of New Varieties of Plants” (hereinafter referred to as the “General Introduction”) and its associated “TGP” documents.

TABLE OF CONTENTS PAGE

1. Subject of these Test Guidelines 3

2. Material Required 3

3. Method of Examination 3

3.1 Duration of Tests 3

3.2 Testing Place 3

3.3 Conditions for Conducting the Examination 3

3.4 Test Design 4

3.5 Number of Plants / Parts of Plants to be Examined 4

3.6 Additional Tests 4

4. Assessment of Distinctness, Uniformity and Stability 4

4.1 Distinctness 4

4.2 Uniformity 4

4.3 Stability 5

5. Grouping of Varieties and Organization of the Growing Trial 5

6. Introduction to the Table of Characteristics 6

6.1 Categories of Characteristics 6

6.2 States of Expression and Corresponding Notes 6

6.3 Types of Expression 6

6.4 Example Varieties 6

6.5 Legend 6

7. Table of Characteristics/Tableau des caractères/Merkmalstabelle/Tabla de caracteres 7

8. Explanations on the Table of Characteristics 14

8.1 Explanations covering several characteristics 14

8.2 Explanations for individual characteristics 14

9. Literature 17

10. Technical Questionnaire 18

1.Subject of these Test Guidelines

These Test Guidelines apply to all varieties of Ocimum basilicum L.

2.Material Required

2.1 The competent authorities decide on the quantity and quality of the plant material required for testing the variety and when and where it is to be delivered. Applicants submitting material from a State other than that in which the testing takes place must ensure that all customs formalities and phytosanitary requirements are complied with.
2.2 The material is to be supplied in the form of seed in the case of seed-propagated varieties or in the form of young rooted plants in the case of vegetatively propagated varieties
2.3 The minimum quantity of plant material, to be supplied by the applicant, should be:
Seed-propagated varieties: 6 g or at least 4000 seeds

Vegetatively propagated varieties: 30 young rooted plants.

2.4 In the case of seed, the seed should meet the minimum requirements for germination, species and analytical purity, health and moisture content, specified by the competent authority. In cases where the seed is to be stored, the germination capacity should be as high as possible and should be stated by the applicant.
2.5 The plant material supplied should be visibly healthy, not lacking in vigor, nor affected by any important pest or disease.
2.6 The plant material should not have undergone any treatment which would affect the expression of the characteristics of the variety, unless the competent authorities allow or request such treatment. If it has been treated, full details of the treatment must be given.

3.Method of Examination

3.1 Duration of Tests

The minimum duration of tests should normally be two independent growing cycles.

3.2 Testing Place

The tests should normally be conducted at one place. If any characteristics of the variety, which are relevant for the examination of DUS, cannot be seen at that place, the variety may be tested at an additional place.

3.3 Conditions for Conducting the Examination

The tests should be carried out under conditions ensuring satisfactory growth for the expression of the relevant characteristics of the variety and for the conduct of the examination.

3.4 Test Design

3.4.1 The design of the tests should be such that plants or parts of plants may be removed for measurement or counting without prejudice to the observations which must be made up to the end of the growing cycle.
3.4.2 In the case of seed-propagated varieties, each test should be designed to result in a total of at least 40 plants, which should be divided between two or more replicates.
3.4.3 In the case of vegetatively propagated varieties, each test should be designed to result in a total of 20 plants, which should be divided between two or more replicates.

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