|DIVESITY OF BLOODMEAL MOSQUITOES (DIPTEA: CULICIDAE) IN THE REPUBLIC OF MOLDOVA
The Center for General and Molecular Biology, Institute of Zoology, Moldova Academy of Sciences
Reviewer: TODERAS Ion., prof. univ., academician of Moldova Academy of Sciences
Key words: Culicidae, diptera, mosquitoes, species diversity, genetic polymorphism
Mosquitoes are the most important group of arthropods adapted for sucking blood from vertebrates. They are distributed all over the world and are of great fundamental, medical and veterinary importance as active bloodsuckers and vectors of many diseases like malaria, yellow fever, encephalitis, filariasis etc. More than half of the world's population lives under the risk of becoming infected by the causative agents of these diseases. Therefore it is very important to investigate the fauna of mosquitoes, their inter- and intraspecific variability on the morphological and genetic levels for successful action against the vectors.
The aim of this investigation was to research fauna of Culicidae mosquitos’ family from Republic of Moldova. Results presented, have been carried out in the Center for General and Molecular Biology, Institute of Zoology, Moldova Academy of Sciences during 2008-2009. The materials were collected from the 17 different regions of Republic of Moldova. Altogether, 24 species from 6 genera of Culicidae family were determinate: Аnopheles hyrcanus (Pallas), А. maculipennis Meig., A. sacharovi Favre, A. plumbeus Stephens, Aedes vexans (Meigen), Ochlerotatus geniculatus (Oliver), O. annulipes (Meigen), O. behningi (Martini), O. caspius (Pallas), O. intrudens (Dyar), O. dianteus (H.D.K), O. excrucians (Walker), O. flavescens (Muller), O. pulchritarsis (Rondani), O. cataphylla (Dyar), O. sticticus (Meigen), Culex modestus Ficalbi, C. pipiens L., C. torrentium Martini, C. territans Walker, Culiseta longiareolata (Macquart), Cs. annulata (Schrank), Coquillettidia richiardii (Ficalbi). Among them 2 species Culiseta longiareolata (vector of beard’s parasites) and Culex torrentium (vector of Ockelbo virus) are specified for the first time for regional fauna.
On the basis of molecular-genetic analyses the two forms of C. pipiens have been distinguished using the flanking region of microsatellite locus CQ11 There was established that for C. pipiens f. molestus the length of amplification product was about 260 pb and for C. pipiens f. pipiens – 190 pb.
Accurate identification is a basic prerequisite to a study of the autecology of a species as well as its biocoenotic relationship in the ecosystem.