Bithell et al. Supplementary material S1




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Bithell et al. Supplementary material

Supplementary material S1 Cultivar seedling heights (1997 seedling data set), nine year old grafted tree rootstock and scion trunk cross sectional area (TCSA) and relationships between seedling height and nine year old TCSA values, for 33 cultivars.

Table S1-1: Northern Territory (NT) accession codes and names of 33 rootstock cultivars, with results for 1997 seedling height (1997 seedling data set), and rootstock and scion trunk cross sectional area (TCSA) from 2006 when trees were 9 years old. Cultivar values are sorted by the scion TCSA rankings. The standard error of the mean (SEM), Tukey’s HSD value and P for the test of equal mean values presented. The 5 rootstock cultivars root sampled in 2010 are shown in bold.

Cultivar name

NT accession code

Seedling height cm

2006 rootstock TCSA cm2

2006 scion TCSA cm2

2006 scion:stock TCSA ratio

Vellaikulamban

51

81.7

306.1

247.9

0.81

Manggadol

68

59.8

323.1

291.3

0.90

Joe Welsh

73

61.0

386.1

340.0

0.89

False Chausa

54

48.2

400.3

352.3

0.89

Manzano

39

66.7

391.4

352.7

0.92

Phoenix

19

64.2

396.1

355.4

0.91

Watertank

14

79.3

410.8

367.0

0.89

Sabre

23

66.7

437.0

371.9

0.86

Kensington Pride

16

70.2

464.4

375.3

0.81

MYP

50

81.2

451.2

380.6

0.84

NT Grain

13

82.2

368.2

381.4

1.04

Okrong

30

67.7

415.0

383.2

0.93

Kimtanya

75

68.0

350.5

385.6

1.09

Nam Doc Mai

36

57.0

350.0

388.0

1.13

Charles

72

63.3

465.9

392.8

0.85

DHC Small

53

83.7

422.7

394.6

0.93

Pancho RH

10

59.7

416.8

435.3

1.05

Pineapple

41

68.0

415.6

438.6

1.06

Cathania

32

67.0

482.9

438.7

0.91

Tong Dum

59

80.2

446.1

448.2

1.01

Banana Callo

35

58.5

508.8

449.0

0.89

Olour

62

59.7

443.7

452.9

1.05

Unknown CPRS

44

65.0

429.2

461.6

1.09

R11T1

58

59.5

463.5

470.7

1.02

Julie Common

34

62.5

504.6

478.2

0.96

Moovandan

61

49.5

546.8

501.9

0.93

Florigon

29

54.3

555.8

508.0

0.93

Bappukai

26

78.7

542.4

508.9

0.94

Pico

40

71.5

459.1

512.1

1.14

Starch

27

52.8

460.7

515.6

1.12

Kurukan

21

67.2

482.6

534.7

1.12

Rosa

60

46.7

667.3

600.0

0.91

Orange

33

48.3

623.1

610.8

0.98

SEM




6.03

30.9

26.7

0.047

HSD




32.95

169.91

146.85

0.257

P




< 0.0001

< 0.0001

< 0.0001

< 0.0001

Table S1-2: Pearson correlation coefficients (r) and P values for correlations of average rootstock cultivar height measurements (1997 seedling data set) against average rootstock or scion TCSA across 33 rootstock cultivars. All rootstock values used in these comparisons are presented in Table S2-1.




r

P

Stock TCSA (mm2)

-0.430

0.0124

Scion TCSA (mm2)

-0.427

0.0132

Stock:scion TCSA ratio

-0.1170

0.5167



Fig. S1-1: Plot of seedling height (1997 seedling data set) verses scion trunk cross sectional area (TCSA) in 2006 when trees were 9 years old for the 33 rootstock cultivars.

Supplementary material S2 Relationships between the height of rootstock seedlings (1997 seedling data set), vigour of orchard trees and root parameters for the five rootstock cultivars root sampled in 2010.

Table S2-1: Linear regression results for the a) height of rootstock seedlings for scion trunk cross sectional area (TCSA) of the rootstock and scion from 2006 and 2010 measurements, rootstock and scion TCSA growth rate (GR), root dry matter (DM) for four root size classes (Class 1 < 0.64 mm (C1); Class 2 0.64-1.88 mm (C2); Class 3 1.88-7.5 mm (C3); Class 4 7.5-23 mm (C4)) and total DM, root DM of each class and total DM relative to rootstock and scion TCSA and b) for orchard trees the 2010 TCSA versus root DM for each class and total DM, and TSCA growth rate for rootstock and scion versus class and total DM or the root DM of each class and total DM relative to rootstock and scion TCSA. Note: superscript stk/sc used to indicate variables that had values for both rootstock (stk) and scion (sc).

Independent variable

Dependant variable

Rootstock

r2/ P-value

Scion

r2/ P-value

a) Rootstock seedlings










Height

TCSA 2006 stk/sc

0.486/0.191

0.599/0.125

Height

TCSA 2010 stk/sc

0.572/0.139

0.500/0.182

Height

TCSA GR stk/sc

0.478/0.196

0.297/0.342

Height

C1 DM

0.024/0.806

-1

Height

C2 DM

0.164/0.499

-

Height

C3 DM

0.015/0.846

-

Height

C4 DM

0.349/0.294

-

Height

Total DM

0.377/0.271

-

Height

C1 DM/TCSA stk/sc

0.013/0.853

0.538/0.158

Height

C2 DM/TCSA stk/sc

0.147/0.525

0.679/0.086

Height

C3 DM/TCSA stk/sc

0.009/0.882

0.553/0.150

Height

C4 DM/TCSA stk/sc

0.715/0.071

0.307/0.332

Height

Total DM/TCSA stk/sc

0.372/0.275

0.072/0.663













b) Orchard trees










TCSA 2010 stk/sc

C1 DM

0.088/0.629

0.012/0.861

TCSA 2010 stk/sc

C2 DM

0.021/0.815

0.001/0.983

TCSA 2010 stk/sc

C3 DM

0.224/0.421

0.122/0.564

TCSA 2010 stk/sc

C4 DM

0.707/0.074

0.540/0.157

TCSA 2010 stk/sc

Total DM

0.766/0.052

0.528/0.165













TCSA GR stk/sc

C1 DM

0.003/0.931

0.044/0.737

TCSA GR stk/sc

C2 DM

0.040/0.748

0.002/0.943

TCSA GR stk/sc

C3 DM

0.020/0.819

0.143/0.530

TCSA GR stk/sc

C4 DM

0.450/0.216

0.610/0.119

TCSA GR stk/sc

Total DM

0.351/0.293

0.490/0.189













TCSA GR stk/sc

C1 DM/TCSA

0.001/0.990

0.889/0.016

TCSA GR stk/sc

C2 DM/TCSA

0.028/0.788

0.764/0.053

TCSA GR stk/sc

C3 DM/TCSA

0.024/0.804

0.461/0.208

TCSA GR stk/sc

C4 DM/TCSA

0.546/0.154

0.001/0.952

TCSA GR stk/sc

Total DM/TCSA

0.353/0.290

0.477/0.197

1no comparison including scion possible



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