Additional file 1: Host proteins associated with Anghv-1 virions as identified by 1D gel/nanolc-ms/ms and 2D nanolc-ms/MS




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Additional file 1: Host proteins associated with AngHV-1 virions as identified by 1D gel/nanoLC-MS/MS and 2D nanoLC-MS/MS.


Categorya

Host proteinb

Species originc

NCBI ID

Predicted molecular mass (kDa)

1D gel/nanoLC-MS/MS

2D nano LC-MS/MS
















Number of peptidesd

Mascot score

Number of peptidese

Mascot score

Cytoskeleton

Actin clone 403

Artemia sp.

113255

42.2

18

431

-

-




Actin depolymerisation factor

Tetraodon nigroviridis

47225287

18.9

1

69

-

-




Alpha-actin

Salmo trutta

8489855

42.2

-

-

10

292




Beta-actin

Anguilla anguilla

82798416

17.8

10

325

-

-






Phoxinus oxycephalus

7546805

42.0

-

-

14

406






Pungitius pungitius

66731680

40.5

-

-

14

405






Rhodeus notatus

10442729

42.0

18

446

-

-




Cytoskeleton-associated protein 5-like

Danio rerio

326680017

93.8

2

44

-

-




Filamin A

Danio rerio

189535920

271.6

5

137

1

55






Ictalurus punctatus

90103410

9.6

1

111

-

-




Profilin-2

Ictalurus furcatus

308321486

13.9

1

73

-

-




Sept2 protein

Danrio rerio

32766415

39.2

1

57

-

-




Simple type II keratin K8a (S1)

Oncorhynchus mykiss

185132941

59.2

6

139

-

-




Slow myotomal muscle tropomyosin

Salmo trutta

3063940

32.7

-

-

1

45

Glycolysis

Aldolase

Ictalurus punctatus

27883578

17.4

1

48

-

-




Glyceraldehyde-3-phosphate dehydrogenase

Anguilla japonica

10567305

32.1

1

61

-

-

Immunological response

Pentraxin

Gasterosteus aculeatus

194277689

23.7

12

93

2

44

Protein glycosylation

Alpha-N-acetylgalactosamine alpha-2,6-sialyltransferase

Takifugu rubripes

5002573

27.3

-

-

5

46

Receptor-like proteins

Alpha-1A adrenoceptor-like

Danio rerio

125823286

53.7

-

-

2

45

Regulatory proteins

Ubiquitin

Salmo sp.

223061

8.5

-

-

1

49




WD repeat domain 1

Danio rerio

37595360

67.1

1

62

-

-

Stress response

Heat shock protein 70

Carassius gibelio

28569550

71.4

11

392

-

-






Ctenopharyngodon idella

323146387

71.7

-

-

5

144






Danio rerio

153792281

70.9

6

256

4

131






Danio rerio

1865782

71.5

10

345

-

-






Oncorhynchus mykiss

17129570

71.3

8

263

-

-






Oreochromis mossambicus

3004463

70.5

12

324

-

-






Pelteobagrus fulvidraco

237688438

71.0

10

348

-

-






Rachycentron canadum

62529292

24.0

5

133

-

-






Scophthalmus maximus

144952758

71.4

10

322

-

-






Silurus meridionalis

126116091

71.1

11

345

-

-






Takifugu rubripes

1620388

70.3

8

264

-

-




Heat shock protein 90

Oncorhynchus tshawytscha

1899173

83.9

1

93

-

-

Transcription-translation control

Sp5 transcription factor-like

Danio rerio

35902791

40.4

6

43

-

-

Transport proteins

Fatty acid binding protein H8-isoform

Chaenocephalus aceratus

2738182

15.0

-

-

2

104




Fatty-acid binding protein heart-type

Cyprinus carpio

281333450

14.7

-

-

2

87




Lipocalin

Tetraodon nigroviridis

47222259

13.4

2

68

-

-




Myelin P2 protein

Osmerus mordax

225708474

15.3

1

69

-

-

Unknown function

Unnamed protein product

Tetraodon nigroviridis

47204907

15.9

-

-

3

47




Unnamed protein product

Tetraodon nigroviridis

47222608

25.3

-

-

1

42




Unnamed protein product

Tetraodon nigroviridis

47210777

20.2

2

52

-

-




Zgc:109744

Danio rerio

71534073

88.7

2

45

-

-

athe host proteins are classified by category and alphabetically ordered, bHomologous protein in different host species are indicated by “, cas a complete A. anguilla sequence was not available, most homologies were found in other species, dnumber of peptides and Mascot score in the complete virion fraction as determined by 1D gel/nanoLC-MS/MS, enumber of peptides and Mascot score in the complete virion fraction as determined by 2D nanoLC-MS/MS



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