Table S1: Activity of fungal endophytes against Plasmodium falciparum (causative agent of malaria) in in vitro assays, organized by host plant family. Data indicate mean percent inhibition of growth (mean % IG) of parasite cells and standard error, the number of plant species and fungal genotypes examined, the number and percent of fungal genotypes that are highly active (i.e. ≥ 50 % IG), and a qualitative statement of activity level. Mean % IG varied significantly between activity levels (F2,137 = 4.67; p = 0.0108).
Table S2: Activity of fungal endophytes against Leishmania donovani (causative agent of leishmaniasis) in in vitro assays, organized by host plant family. Data indicate mean percent inhibition of growth (mean % IG) of parasite cells and standard error, the number of plant species and fungal genotypes examined, the number and percent of fungal genotypes that are highly active (i.e. ≥ 50 % IG), and a qualitative statement of activity level. Mean % IG varied significantly between activity levels (F2,194 = 4.86; p = 0.0087).
Family (Order)
Mean %IG (± SE)
Plant Species Examined
Fungal Genotypes Examined
Highly Active Genotypes
% Highly Active Genotypes
Activity Level
Bignoniaceae (Scrophulariales)
24.8 (9.2)
3
6
0
0
High
Rubiaceae (Gentianales)
21.9 (4.1)
10
25
2
8
High
Apocynaceae (Gentianales)
20 (5.3)
3
17
2
11.8
High
Verbenaceae (Lamiales)
17.6 (4.7)
5
22
3
13.6
Moderate
Araceae (Alismatales)
15.8 (7.5)
4
9
1
11.1
Moderate
Moraceae (Rosales)
15.1 (4.6)
5
20
2
10
Moderate
Fabaceae (Fabales)
14.9 (3.2)
15
41
4
9.8
Moderate
Euphorbiaceae (Malpighiales)
9.4 (6.2)
3
12
0
0
Low
Clusiaceae (Malpighiales)
6.2 (6)
3
13
0
0
Low
Annonaceae (Magnoliales)
4.4 (7.1)
3
9
0
0
Low
Table S3: Activity of fungal endophytes against Trypanosoma cruzi (causative agent of Chagas’ disease) in in vitro assays, organized by host plant family. Data indicate mean percent inhibition of growth (mean % IG) of parasite cells and standard error, the number of plant species and fungal genotypes examined, the number and percent of fungal genotypes that are highly active (i.e. ≥ 50 % IG), and a qualitative statement of activity level. Mean % IG varied significantly between activity levels (F1,181 = 4.20; p = 0.0428).
Family (Order)
Mean %IG (± SE)
Plant Species Examined
Fungal Genotypes Examined
Highly Active Genotypes
% Highly Active Genotypes
Activity Level
Fabaceae (Fabales)
20.8 (2.3)
15
39
6
15.4
High
Rubiaceae (Gentianales)
20.8 (3.1)
10
23
3
13.1
High
Bignoniaceae (Scrophulariales)
20.6 (6.7)
3
6
0
0
High
Araceae (Alismatales)
19.8 (5.5)
4
9
1
11.1
Moderate
Apocynaceae (Gentianales)
17.8 (3.9)
3
17
1
5.9
Moderate
Euphorbiaceae (Malpighiales)
16.8 (4.9)
3
11
0
0
Moderate
Clusiaceae (Malpighiales)
15.5 (4.7)
3
12
0
0
Moderate
Verbenaceae (Lamiales)
15.3 (3.3)
5
22
1
4.5
Moderate
Moraceae (Rosales)
15.1 (3.8)
5
16
1
6.3
Moderate
Annonaceae (Magnoliales)
10.9 (5.8)
3
8
0
0
Moderate
Table S4: Activity of fungal endophytes against MCF-7 breast cancer cells in in vitro assays, organized by host plant family. Data indicate mean percent inhibition of growth (mean % IG) of parasite cells and standard error, the number of plant species and fungal genotypes examined, the number and percent of fungal genotypes that are highly active (i.e. ≥ 50 % IG), and a qualitative statement of activity level. Mean % IG varied significantly between activity levels (F2,171 = 4.49; p = 0.0125).