Table A1
|
(continued)
|
|
Pop. no.
|
Voucher numbers (GenBank accession numbers AM18-)
|
Geographic origin
|
Haplotypes 12S, [16S]
|
035
|
431, 888-892
|
P, Almodovar
|
S.Iberia 1
|
036
|
438, 883-887
|
P, Castro Verde
|
S.Iberia 1
|
037
|
1976-1981
|
P, Castro Verde
|
S.Iberia 1, 23
|
038
|
443, 893-897
|
P, Ourique
|
S.Iberia 1, 8, 16
|
039
|
433, 1912-1916
|
P, Beja
|
S.Iberia 1
|
040
|
1950, 1951
|
S, Doñana National Park
|
S.Iberia 1
|
041
|
3184
|
S, Doñana National Park
|
S.Iberia 3
|
042
|
1952-1954
|
S, Doñana National Park
|
S.Iberia 2, 3
|
043
|
559, 992, 996
|
S, Doñana National Park
|
S.Iberia 3, 17
|
044
|
416
|
S, N. Doñana Natural Park
|
S.Iberia 2
|
045
|
3182
|
S, Doñana National Park
|
S.Iberia 17
|
046
|
3183
|
S, N. Doñana Natural Park
|
S.Iberia 3
|
047
|
(3886-3888, 4183)
|
S, Villamanrique
|
S.Iberia 4, [1]
|
048
|
475
|
S, Villamanrique
|
S.Iberia 4
|
049
|
412
|
S, Villamanrique
|
S.Iberia 4
|
050
|
777
|
S, Villamanrique
|
S.Iberia 4
|
051
|
972
|
S, Villamanrique
|
S.Iberia 4
|
052
|
973
|
S, Villamanrique
|
S.Iberia 7
|
053
|
66, (3883-3885)
|
S, Utrera
|
S.Iberia 3, [1]
|
054
|
476, 477
|
S, El Cuervo
|
S.Iberia 13
|
055
|
415
|
S, Fuentes de Andalucía
|
S.Iberia 1
|
056
|
165, (3913-3915)
|
S, Badajoz
|
S.Iberia 11, [1]
|
057
|
159, 4982, 4983, (3906, 3908, 3909)
|
S, Navalvillar de Pela
|
S.Iberia 7, 10, [1]
|
058
|
162, (3910-3912)
|
S, Cáceres
|
S.Iberia 12, [1]
|
059
|
(3907, 3916, 3917, 4184)
|
S, Cáceres
|
S.Iberia 12, [1]
|
060
|
441
|
P, Mértola
|
S.Iberia 6
|
061
|
442, 927
|
P, Mértola
|
S.Iberia 1
|
062
|
435
|
P, Castro Verde
|
S.Iberia 5
|
063
|
437
|
P, Castro Verde
|
S.Iberia 5
|
064
|
439
|
P, Castro Verde
|
S.Iberia 6, [1]
|
065
|
436, 944-946
|
P, Castro Verde
|
S.Iberia 6, [1]
|
066
|
430
|
P, Almodovar
|
S.Iberia 1
|
067
|
4986
|
P, Almodovar
|
S.Iberia 1
|
068
|
4990
|
P, Beja
|
S.Iberia 1
|
069
|
434
|
P, Beja
|
S.Iberia 1
|
070
|
1927-1929
|
P, Beja
|
S.Iberia 1
|
071
|
4989
|
P, Beja
|
S.Iberia 1
|
072
|
432
|
P, Beja
|
S.Iberia 1, [3]
|
073
|
4988
|
P, Beja
|
S.Iberia 1
|
074
|
4984
|
P, Faro do Alentejo
|
S.Iberia 1
|
075
|
4985
|
P, Faro do Alentejo
|
S.Iberia 1
|
076
|
1924-1926
|
P, Cuba
|
S.Iberia 1
|
077
|
4991
|
P, Cuba
|
S.Iberia 1
|
078
|
4987
|
P, Alvito
|
S.Iberia 1
|
079
|
440
|
P, Ferreira do Alentejo
|
S.Iberia 1
|
080
|
4996, 4997
|
P, Estremoz
|
S.Iberia 1
|
081
|
4998-5000
|
P, Estremoz
|
S.Iberia 1
|
082
|
425, 825-829, 1970-1975
|
S, Tarifa
|
Cádiz 1, [1]
|
083
|
488, 730-734
|
S, Barbate
|
Cádiz 1, [1]
|
Table A1
|
(continued)
|
|
Pop. no.
|
Voucher numbers (GenBank accession numbers AM18-)
|
Geographic origin
|
Haplotypes 12S, [16S]
|
084
|
485, 725-729
|
S, Tahivilla
|
Cádiz 1, 2
|
085
|
486, 720-724
|
S, Benalup
|
Cádiz 2, 5, [1, 2]
|
086
|
421, 478, 908-911
|
S, Puerto Real
|
Cádiz 4, [1]
|
087
|
1988-1992
|
S, El Puerto de Sta. María
|
Cádiz 5
|
088
|
420
|
S, Tarifa
|
Cádiz 1, [1]
|
089
|
487, 735-737
|
S, Tahivilla
|
Cádiz 1
|
090
|
484, 719
|
S, Benalup
|
Cádiz 1, 5
|
091
|
1934-1937
|
S, Conil de la Frontera
|
Cádiz 5
|
092
|
551
|
S, San Fernando
|
Cádiz 5, [3]
|
093
|
1933
|
S, Puerto Real
|
Cádiz 3
|
094
|
76, 1961, 1962, 1964, 1965, (3889-3893, 4181)
|
P, Sagres
|
Portugal 1, [P]
|
095
|
447, 898-902
|
P, Sagres
|
Portugal 1, 5, [P]
|
096
|
446, 878-882
|
P, Tunes
|
Portugal 2, 3, [P]
|
097
|
445, 937-941
|
P, Faro
|
Portugal 4, [P]
|
098
|
1966-1969
|
P, Vila do Bispo
|
Portugal 1
|
099
|
448, 1941, 1942
|
P, Vila do Bispo
|
Portugal 1
|
100
|
1938-1940
|
P, Vila do Bispo
|
Portugal 1
|
101
|
449, 1993, 1995
|
P, Vila do Bispo
|
Portugal 1
|
102
|
634, 635, 4992-4995
|
S, La Albuera
|
Gitanilla 1, 2
|
103
|
(3881, 3882, 4182)
|
S, La Albuera
|
Gitanilla 1, [G]
|
104
|
(3871, 3872, 4174)
|
M, Ain-Benimathar
|
T. m. s. 1, [3]
|
105
|
(3868-3870, 4173)
|
Tunisia, Jendouba
|
T. m. s. 3, [1]
|
106
|
(3865, 3866, 4172)
|
Tunisia, Kairouan
|
T. m. s. 2, [2]
|
107
|
(3862-3864)
|
Tunisia, Tunis
|
T. m. s. [1]
|
108
|
(3873-3875, 4177)
|
M, El-Hajeb
|
T. m. m. 2, [6]
|
109
|
(3904, 3905, 4179)
|
M, Mrirt
|
T. m. m. 1, [5]
|
110
|
(3876, 3879, 4176)
|
M, Timahdite
|
T. m. m. 1, [7]
|
111
|
1930-1932
|
M, Timahdite
|
T. m. m. 1
|
112
|
(3877, 3878)
|
M, Rabat, pond 59
|
T. m. m. [7]
|
113
|
(3880)
|
M, Rabat, pond 60
|
T. m. m. [7]
|
114
|
(3894, 4175)
|
M, Casablanca, pond 002
|
T. m. m. 3, [1]
|
115
|
(3897, 3898)
|
M, Casablanca, pond 005
|
T. m. m. [1]
|
116
|
(3895, 3896)
|
M, Safi
|
T. m. m. [2]
|
117
|
(3899, 3900, 4178)
|
M, Essaouira, pond 049
|
T. m. m. 4, [2]
|
118
|
(3901)
|
M, Essaouira, pond 050
|
T. m. m. [3]
|
119
|
(3902, 3903, 4180)
|
M, High Atlas
|
T. m. m. 5, [4]
|
120
|
3333
|
France, Crau
|
T. c. c. 4
|
121
|
(3824-3826)
|
Germany, Ingolstadt
|
T. c. c. [1]
|
122
|
(3851-3853, 4171)
|
Tunisia, Jendouba
|
T. c. c. 4, [3]
|
123
|
(3833-3835)
|
Sicily, Custonaci
|
T. c. c. [3]
|
124
|
(3828)
|
Favignana Island, pond 1
|
T. c. c. [1]
|
125
|
(3846, 3847)
|
Serbia, Melenci, pond 1
|
T. c. c. [2]
|
126
|
(3848-3850, 4170)
|
Serbia, Melenci, pond 2
|
T. c. c. 4, [1]
|
127
|
(3841, 4167)
|
Hungary, Tiszabercel
|
T. c. c. 4, [5]
|
128
|
(3821-3823, 4166)
|
Austria, commercial kit
|
T. c. c. 1, [6]
|
129
|
(3843-3845, 4168, 4169)
|
Russia, Uljanowsk
|
T. c. c. 1, [7]
|
130
|
(3827, 4165)
|
UAE, Sharjah
|
T. c. c. 2, [6]
|
Fig. A1 ML tree based on COI sequences (RAxML program, setting
‘estimate proportion of invariable sites’; best evolutionary model obtained by Modeltest was TrN+I+G, selected by AIC). ML bootstrap support (obtained with RAxML) given for selected branches. Outgroups [GenBank sequences of Lepidurus apus (accession number EF189669), L. arcticus (AF209067), L. couesii (DQ310622), L. lemmoni (GQ144447), Triops longicaudatus (DQ310623 and GQ144444), T. australiensis (DQ889135), T. granarius (GQ144446)] removed for clarity. Samples labelled, as applicable, with short names of main phylogenetic lineages (Table A1) followed by museum specimen tissue voucher numbers (MTD-TW; sequences submitted to GenBank, acc. nrs. FN691430–FN691444) or by GenBank accessions, or labelled with GenBank accessions containing numbers but no lineage data (samples with GenBank taxon labels apparently resulting from erroneous species identification, i.e. samples submitted to GenBank with invalid species names). Abbreviations: T.c. = Triops cancriformis; T.m. = T. mauritanicus
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|