Symbiovar loti genes are widely spread among Cicer canariense mesorhizobia, resulting in symbiotically effective strains




Дата канвертавання17.04.2016
Памер12.15 Kb.
Symbiovar loti genes are widely spread among Cicer canariense mesorhizobia, resulting in symbiotically effective strains
Pilar Martínez-Hidalgo, Juan Pérez-Yépez, Encarna Velázqueza, Ricardo Pérez-Galdonab, Eustoquio Martínez-Molinaa and Milagros León-Barriosa*
aDepartamento de Microbiología y Genética. Universidad de Salamanca. 37007 Salamanca. Spain. bDepartamento de Bioquímica, Microbiología, Biología Celular y Genética. Universidad de La Laguna. 38071 La Laguna. Tenerife. Canary Islands. Spain.
¥These two authors have equally contributed to this work

*Corresponding author: Milagros León Barrios. Departamento de Microbiología y Biología Celular. Universidad de La Laguna. Facultad de Farmacia. Avd. AstrofísicoFco. Sánchez s/n. Tel: 34922318481; Fax: 34922318666; email:mileonba@ull.es




Figure S1. Maximum likelihood tree (WAG+G) based on nifH amino acid sequences (218 positions, from amino acid 36 to 255, referring to the nifH gene of Mesorhizobium MAFF303099 strain) of Cicer canariense isolates and reference strains. The significance of each branch is indicated by a bootstrap value (≥50%) calculated for 500 subsets. The scale bar indicates the number of substitutions per site.


Figure S2. Eight week plants of C. canariensis inoculated with Cicer canariense mesorhizobial strains CCANP122, CCANP14 and CCANP33, and the reference strains Mesorhizobium ciceri USDA 3383T and Mesorhizobium loti NZP2213T. N-supplemented plants were watered with Rigaud and Puppo nutrient solution with 4 mM KNO3. Negative control corresponds to uninoculated plants watered with N-free nutrient solution. C. canariense nodules from eight week plants inoculated with M. loti NZP2213T (A), M. ciceri USDA 3383T (B), CCANP122 (C), CCANP14 (D) and CCANP33 (E).




A

B

Figure S3. Four week plants of L. corniculatus inoculated with three Cicer canariense mesorhizobial strains (CCANP122, CCANP14 and CCANP33) and the reference strain Mesorhizobium loti NZP2213T. (A) Average of the nodule number. Bars indicate Standard error. (B) L. corniculatus nodules from plants inoculated with M. loti NZP2213T (A), CCANP122 (B), CCANP14 (C) and CCANP33 (D).





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