Supporting information to




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Supporting information to:

Phytoextraction of uranium from contaminated soil by Macleaya cordata before and after application of EDDS and CA

Journal name: Environmental Science and Pollution Research

Chang-wu Li, Nan Hu, De-xin Ding*, Jin-song Hu, Guang-yue Li, Yong-dong Wang

Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China.

*Corresponding author: De-xin Ding

Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, 28 West Changsheng Road, Hengyang, Hunan 421001, People’s Republic of China.

Telephone: +86 734 8160533.

Fax: +86 734 8160533

E-mail: dingdxzzz@163.com; nhdxlcw@163.com (Ding, D. X.).

Chang-wu Li and Nan Hu are contributed equally to this work.

This supporting information contains four (4) Online Resource.



Online Resource 1 Chemical and physical properties of tailing soil and campus soil after incubation

Parameter

Unit

Value

Tailing soil

Campus soil

Clay (%) < 0.002 mm




13.5

25.1

Silt (%) 0.002- 0.02 mm




13.6

25.8

Sand (%) > 0.02 mm




72.9

49.1

Soil texture a




Loam

Clay loam

pH (in H2O) b




6.49 (0.02)

8.14 (0.03)

Organic carbon c

%

1.43 (0.08)

2.11 (0.01)

Calcium carbonate equivalent d

%

0.63 (0.02)

1.14 (0.03)

Oxalate extractable e

mg kg-1







Fe




1463 (34)

1746 (48)

Al




165 (5)

218 (7)

Cation exchange capacity f

cmol kg-1

11.00 (0.15)

21.18 (0.28)

Exchangeable cations g

cmol kg-1







Ca




14.49 (0.67)




Mg




1.47 (0.02)




K




0.53 (0.01)




Na




0.61 (0.01)




Soil moisture h

%

1.64 (0.01)

3.45 (0.01)

Saturation moisture content i

%

32.48 (1.80)

39.35 (0.46)

Total uranium concentration

mg kg-1

18.0 (0.1)

18.0

a Robinson’s pipette method;

b Measured at a 1:2.5 soil-to-water ratio;

c Potassium dichromate oxidation spectrophotometric method;

d Back titration of an excess of HCl with NaOH;

e (NH4)2C2O4 extraction;



f 1 M NH4OAc (pH 7) method, NaOAc (pH 8.2) method;

g 1 M NH4OAc extractable cations;

h Gravimetric method (105°C);

i Cutting ring method.

Online Resource 2 Relationship between DOC concentration and uranium concentration in soil solution during the cultivation period






Online Resource 3 Influence of chelate on uranium concentration in shoot of Macleaya cordata. Values annotated with different letters have significant difference (Tukey's multiple range test with P < 0.05)

Online Resource 4 Influence of chelate on Fe and Mn concentrations in soil solution

Treatment

Before application

of chelate



Time after application of chelate (days)

1

3

5

7

10

14




Fe concentration (mg L−1)

Control

0.3 (0.1) a

0.4 (0.1) a

0.4 (0.0) a

0.3 (0.1) a

0.3 (0.0) a

0.2 (0.0) a

0.2 (0.1) a

1.0 mmol kg-1 EDDS

0.3 (0.1) b

4.9 (1.7) ab

9.5 (2.9) a

10.1 (4.2) a

9.0 (3.2) a

6.6 (3.7) ab

5.0 (1.8) ab

2.5 mmol kg-1 EDDS

0.3 (0.1) b

8.6 (2.2) a

23.0 (4.6) a

21.6 (5.6) a

23.2 (7.1) a

24.0 (7.4) a

16.8 (4.9) a

5.0 mmol kg-1 EDDS

0.3 (0.1) b

10.5 (4.1) ab

26.3 (11.6) a

26.4 (12.0) a

20.6 (4.0) a

16.1 (4.9) ab

9.5 (2.5) ab

10.0 mmol kg-1 EDDS

0.3 (0.1) b

23.1 (4.5) a

38.4 (12.5) a

33.3 *

31.1 *

33.1 *

37.1 *

1.0 mmol kg-1 CA

0.3 (0.1) c

7.6 (2.1) a

2.9 (0.8) b

0.5 (0.1) c

0.5 (0.0) bc

0.3 (0.1) c

0.3 (0.0) c

2.5 mmol kg-1 CA

0.3 (0.1) c

18.5 (2.7) a

8.6 (4.4) b

1.4 (0.8) c

0.7 (0.3) c

0.2 (0.0) c

0.2 (0.0) c

5.0 mmol kg-1 CA

0.3 (0.1) c

51.0 (25.4) a

34.4 (16.7) ab

7.7 (2.2) bc

1.9 (1.2) c

0.3 (0.1) c

0.2 (0.1) c

10.0 mmol kg-1 CA

0.3 (0.1) c

101.3 (39.2) ab

159.5 (44.9) a

60.3 (17.7) bc

14.5 (7.0) c

0.9 (0.5) c

0.3 (0.1) c




Mn concentration (mg L−1)

Control

0.11 (0.03) a

0.10 (0.03) a

0.11 (0.01) a

0.05 (0.02) a

0.08 (0.04) a

0.07 (0.01) a

0.07 (0.01) a

1.0 mmol kg-1 EDDS

0.11 (0.03) b

5.38 (1.67) a

1.03 (0.41) b

0.39 (0.22) b

0.21 (0.19) b

0.09 (0.04) b

0.10 (0.01) b

2.5 mmol kg-1 EDDS

0.11 (0.03) c

34.73 (8.15) a

22.63 (7.85) b

8.28 (0.83) bc

3.74 (1.43) c

0.83 (0.21) c

0.11 (0.04) c

5.0 mmol kg-1 EDDS

0.11 (0.03) c

44.45 (17.32) a

40.55 (18.64) a

32.80 (16.16) ab

15.57 (6.10) abc

5.20 (2.46) bc

2.35 (1.21) bc

10.0 mmol kg-1 EDDS

0.11 (0.03) b

121.02 (21.10) a

164.49 (71.03) a

146.29 *

79.89 *

110.12 *

118.32 *

1.0 mmol kg-1 CA

0.11 (0.03) b

1.39 (0.26) a

0.21 (0.17) b

0.09 (0.06) b

0.12 (0.04) b

0.24 (0.29) b

0.08 (0.01) b

2.5 mmol kg-1 CA

0.11 (0.03) b

10.79 (5.30) a

0.17 (0.06) b

0.10 (0.02) b

0.09 (0.03) b

0.14 (0.10) b

0.09 (0.05) b

5.0 mmol kg-1 CA

0.11 (0.03) b

31.68 (17.86) a

3.99 (1.63) b

1.36 (0.38) b

0.98 (0.55) b

0.68 (0.35) b

0.41 (0.32) b

10.0 mmol kg-1 CA

0.11 (0.03) c

56.85 (24.28) a

41.01 (17.87) ab

11.68 (6.41) bc

1.52 (0.76) c

0.37 (0.24) c

0.15 (0.09) c

For each chelate, mean values marked with different letters have significant differences between the times of sampling at the P < 0.05 level according to the Tukey's multiple range test. SD is indicated in bracket following the value (n = 3). * means no Tukey multiple range test was conducted (only one replicate)



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