Some research in Japan resulted that methanol extract of livewort (Marchantia polymorpha L.) showed anticancer activity against kb cell




Дата канвертавання22.04.2016
Памер5.11 Kb.
ABSTRACT
Some research in Japan resulted that methanol extract of livewort (Marchantia polymorpha L.) showed anticancer activity against KB cell. Other species of genus Marchantia, which are Marchantia palaceae Bertol var diptera (Mont) and Marchantia tosana Steph. also showed the same activity. Based on similarity of the genus, this research will determine anticancer activity of livewort (Marchantia cf. planiloba Steph.).
The ether extract and methanol extract of livewort (Marchantia cf. planiloba Steph.) were tested for its anticancer activity using viability test method on cell culture of mice myeloma. The myeloma cell were incubated in RPMI media contained 10 % of Fetal Bovine Serum (FBS). The concentration of ether extract and methanol extract of livewort (Marchantia cf. planiloba Steph.) were 1000 ppm, 100 ppm, 10 ppm and 1 ppm. Etoposide was used as possitive control in three different concentration (100 ppm, 10 ppm and 1 ppm) while RPMI 1640 was used as negative control. Their solution were added into myeloma cell culture in microwell plate and then incubated for 24 hours, at 37oC in CO2 incubator. Determination of anticancer activity used trypan blue exclusion method, with parameter value was viability of myeloma cell.
Analysis of varian of the data showed that ether extract and methanol extract of livewort (Marchantia cf. planiloba Steph.) have anticancer activity against cell culture of mice myeloma. Probit analysis of the data indicates the LC50 of ether extract of (Marchantia cf. planiloba Steph.) was 163,21 ppm and methanol extract of (Marchantia cf. planiloba Steph.) was 358,95 ppm. Thin layer chromatography was used to identify the phytochemical content of livewort (Marchantia cf. Planiloba Steph.). The thin layer chromatogram profile suggested that ether and methanol extract of livewort (Marchantia cf. Planiloba Steph.) contains terpenoid or steroid compounds.
Keywords : Ether extract and methanol extract of livewort (Marchantia cf. planiloba Steph.), cell culture of mice myeloma, anticancer, viability test.


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