S emily Quenneville, Margarette Youssef, Danielle Del Greco ertoli Cell Factsheet




Дата канвертавання22.04.2016
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S
Emily Quenneville, Margarette Youssef, Danielle Del Greco
ertoli Cell Factsheet

Estela M. Munoz, Teresa Fogal, Susana Dominguez, Luis Scardapane, and Ramon S. Piezzi

Ultrastructural and Morphometric Study of the Sertoli Cell of the Viscacha (Lagostomus maximus maximus) During the Annual Reproductive Cycle. The Anatomical Record (2001) 262:176–185.125
Objective of the study:

The main focus of this experiment was to study the morphological changes within the Sertoli cells of viscacha (Lagostomus maximus maximus) throughout their annual reproductive cycle. Specifically, they concentrate on determining the structural parameters that are more sensitive to hormonal fluctuations.



Assumptions: The authors assumed that seasonal endocrine fluctuations and local factors could be involved in the regulation of Sertoli cells, in which they are not just a response to photo-period-induced endocrine fluctuations.
Methods:

Thirty males were captured from the same geographical region, anesthetized, and decapitated. Each left testis was removed and sliced, fixed, dehydrated, embedded, and finally stained. These methodologies provided slides for light microcopy. Further sectioning and staining was utilized to create slides for electron microscopy. All specimens were collected from Stages III and IV of the spermatogenic cycle. To determine the number of Sertoli cells per seminiferous tubule, paraffin-embedded sections were counted. Sertoli cell morphology was assessed at the electron microscope level (10000-32700x) at three different periods within the reproductive cycle. Proposed flaws of these methods include the lack of consideration for the rough endoplasmic reticulum because of its small size, and the missing measurement of the ER and Golgi apparatus from the regressed testes because of excessive dilatation of their components. These employed methods were applicable and appropriate in order to accomplish the outlined objectives.


Results:

This section extensively outlines the differences that occurred within the Sertoli cell during the period of maximum testicular activity in opposition to the quiescent period. During the active period, several well-developed Golgi complexes proliferated along with extensive desmosome-like junctional complexes between neighbouring Sertoli cells. The Sertoli cell number per seminiferous tubule cross section was 15.24 + /- 0.15. During the quiescence period, the size of the Sertoli cell and organelle content were reduced. The cytoplasm contained many clear vacuoles, which is related to seminiferous tubule damage and correlated with a pronounced dilatation of the ER and Golgi complex and other organelles. An increase in lipid inclusions and dense bodies in the basal cytoplasm occurred. Junctional complexes were short, tortuous, and frequently interrupted. The Sertoli cell per seminiferous tubule cross section is 9.53 +/- 0.15. Within the period of testicular recovery, Sertoli cells gradually recovered their nuclear and cytoplasmic characteristics. The dominant organelles in this stage were dense bodies, however experienced poorly developed Golgi complexes. The Sertoli cell per seminiferous tubule was 10.08 +/- 0.7.



Discussion:

The discussion clearly iterates conclusions derived from the achieved results. That is, overall, viscacha Sertoli cells undergo marked nuclear and cytoplasmic changes during the annual reproductive cycle which lead to functional and morphological differences throughout the cycle. The results further allow the authors to postulate that seasonal changes noted in the Sertoli cells of Lagostomus maximus maximus are not just a response to photoperiod-induced endocrine fluctuations. Rather, it is likely that several local factors are involved in this process. These deductions are logically retrieved and valid based on the detailed study of the Sertoli cells at three stages during the cycle. The shortcomings of the paper were briefly discussed. The authors highlight that the degree of regression and recovery exhibited by the viscacha Sertoli cell is closely related to that shown by the associated germ cells. This observation leads to assumptions that the seasonal changes noted in the Sertoli cells of Lagostomus maximus



maximus are not just a response to photoperiod-induced endocrine fluctuations. Rather, it is likely that several local factors are involved in this process. Therefore, regional and stage-dependent morphological changes need to be further investigated.
Effectiveness of Abstract:

This paper has presented many detailed results of the morphology of the Sertoli cell through the reproductive cycle and thus at times is very wordy. However, the abstract is effective at summarizing these extensive results. It briefly discusses the paper as a whole but remains detailed enough to provide us with a precise picture of what the paper entails.


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