|Quantification of pollen aeroallergens dispersed in the atmosphere of different Spanish bioclimatic areas in 2007
Rodriguez-Rajo, F. Javier 1, Vega-Maray, Ana 2, González-Parrado, Zulima 2, Moreno-Grau, Stella 3, Elvira-Rendueles, Belén 3, Jato, Victoria 1, Fernández-González, Delia 2, Seoane-Camba, Juan 4, Suárez-Cervera, María 4.
1 Dept. Plant Biology and Soil Sciences, Sciences Faculty of Ourense, University of Vigo, Ourense, Spain; 2 Dept. of Biodiversity and Environmental Management, Biological and Environmental Sciences Faculty, University of Leon, Leon, Spain; 3 Dept. of Environmental and Chemical Engineering, Technical University of Cartagena, Cartagena, Spain; 4 Dept. of Botany, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain.
Allergies caused by airborne proteins are nowadays on the rise. In cold temperate climates, we can recognize proteins with enzymatic activity such as lipid transport proteins, LTPs (Par j 1-2), proteins involved in the carbohydrate metabolism (Cup a 1, Pla a 1 and Pla a 2) and expansins (Lol p1) among the most common allergens inducing allergy and asthma episodes.
Few aerobiological allergic asthma epidemiologic studies are focused on aeroallergen quantification as most of them only consider pollen counts. As a result, the purpose of this research is to evaluate the interrelationship between pollen counts and the atmospheric aeroallergen concentrations in different Spanish bioclimatic areas by considering the different common pollinosis sources from each area. The taxa Platanus, Poaceae and Urticaceae were selected, in Ourense (Northwest, suboceanic climate), Poaceae in León (Northwest, continental climate), and Platanus, Olea and Urticaceae in Cartagena (Southeast, Mediterranean climate).
The analysis was carried out using three Hirst-type volumetric samplers and three Burkard cyclone samplers working simultaneously during the year 2007. Conventional pollen counts were based on microscopy identification techniques. The enzyme-linked immunosorbent assay (ELISA) double sandwich modified technique was applied for the measurement of the aeroallergens. Antibodies to recognize Ole e 1, Par j 1-2, Pla a 1, Pla a 2 and group 1 of grass antigens were essayed to determine the concentration of the pollen proteins in the atmosphere. This gave us a quantifiable parameter and a reference in the simultaneous detection of pollen counts and improved the sampling and analysis.
The result of this research demonstrates that daily aeroallergen activity follows the variations of the daily mean pollen concentrations curve in most cases. The Spearman correlation analysis test showed the most important relationship between Lol p1 allergen quantification and Poaceae pollen counts, while the lower values were registered for Urticaceae. In the Northwest suboceanic area, pollen from Urticaceae was separated into two groups (Urtica membranacea type and Parietaria type). Only when data from Parietaria pollen type was correlated with Par j1-2 allergen atmospheric concentrations, the Spearman coefficient value and its probability rose. Finally, the Platanus pollen concentrations presented very significant correlations with Pla a1 aeroallergens only in the Northwest suboceanic area.
The combination of pollen counts and the quantification of airborne proteins allows a more reliable assessment of allergen exposure. The application of this specific and quantitative antigen-antibody technique to control the content of allergens in the air represents an important advance in the epidemiologic study of allergic respiratory diseases.
Grants CGL2006-15103-C04-01,02,03,04 from the Department of Education and Science of Spain.
CORRESPONDENCE: F. Javier Rodríguez-Rajo, e-mail email@example.com; tel.
+34 988387193, Fax +34 988397001. (Poster presentation)