Pharmacognostical, phytochemical and antimicrobial activity




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PHARMACOGNOSTICAL, PHYTOCHEMICAL

AND ANTIMICROBIAL ACTIVITY

ON

Jasminum grandiflorum Linn


SYNOPSIS FOR


M.PHARM DISSERTATION
SUBMITTED TO

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES

BY

Sandeep
Department of Pharmacognosy


THE OXFORD COLLEGE OF PHARMACY
BANGALORE-78

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES, KARNATAKA, BANGALORE

ANNEXURE-II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION

1.

Name of the candidate and Address

Sandeep


The Oxford College of Pharmacy,

J.P. Nagar, Ist Phase,

Bangalore-560078.

PERMANENT ADDRESS:

S/o Shri Satya Prakash / Smt. Om Devi

C/o Om Trading Co./ Sandeep studio

Gatatree Nagar, Main Road Pukhrayan

Kanpur (Dehat) U.P. INDIA -209111.



2.

Name of the Institution

The Oxford College of Pharmacy, J.P.Nagar, Ist Phase,

Bangalore-560078.



3.

Course of study and subject

M.Pharm, Pharmacognosy




4.

Date of Admission

16/04/2007


5.

Title of the Topic

PHARMACOGNOSTICAL, PHYTOCHEMICAL AND ANTIMICROBIAL ACTIVITY ON



Jasminum grandiflorum Linn




6.




Brief resume of the intended work:

6.1- Need for the study:

Leaf of Jasminum grandiflorum is also called as Chameli, Chambeli and Mallige belonging to family Oleaceae. It is a plant with fragrant flowers is generally found in all over India, especially in the temperate regions and on the temperate Himalayas(1).

The Chameli leaves contain resin, salicylic acid, jasminine an alkaloid and an astringent principle(2). An Indole oxygenase has been isolated from the leaves(3). Secoiridoid glucosides, 2″-epifraxamoside and demethyl-2″-epifraxamoside, and the secoiridoid, jasminanhydride were isolated from Jasminum grandiflorum Secoiridoid glucosides, 2″-epifraxamoside and demethyl-2″-epifraxamoside, and the secoiridoid, jasminanhydride were isolated from Jasminum grandiflorumSecoiridoid glucosides, 2″-epifraxamoside and demethyl-2″-epifraxamoside, and the secoiridoid, jasminanhydride were isolated from Jasminum grandiflorum Secoiridoid glucosides viz., 2″-epifraxamoside and demethyl-2″-epifraxamoside, jasminanhydride were isolated from Jasminum grandiflorum(4) and the leaves also have reported to contain ascorbic acid, anthranilic acid and its glucoside(5). Terpenoids viz., benzyl acetate, benzyl benzoate, jasmine, linalool and phytol isomers have been reported in the flowers(6), Also Oleacein (ACE inhibitor), 2-(3,4-dihydroxyphenyl)ethanol, isoquercitrin and ursolic acid(7) has been isolated from aerial parts.

The leaves of Jasminum grandiflorum is used in folk medicine for treating ulcerative stomatitis, toothache, skin diseases, ulcers, wounds, corns and also as gargles(9). It has been reported to possess anti ulcer in vitro antioxidant(10), anti-lipid per oxidation (11) and spasmolytic activity(12). Secoiridoid glucosides, 2″-epifraxamoside and demethyl-2″-epifraxamoside, and the secoiridoid, jasminanhydride were isolated from Jasminum grandiflorum

Although very less emphasis has been given to pharmacognostical, phytochemical and pharmacological studies. Therefore it is proposed to study the pharmacognostical properties of leaves of Jasminum grandiflorum, isolation of chemical constituents and antimicrobial activity of extract and isolated compound.
6.2 – Review of Literature:


  1. An alkaloid jasminine, resin and salicylic acid have been reported from the leaves of Chameli(2).

  2. An Indole oxygenase from the leaves of Jasminum grandiflorum(3).

  3. Secoiridoid glucoside has been isolated from Jasminum grandiflorum(4).

  4. The leaves of Chameli contain ascorbic acid, anthranilic acid and its glucoside(5).

  5. Terpenoids viz., benzyl acetate, benzyl benzoate, jasmine, linalool and phytol isomers have been reported in the flowers(6).

  6. Oleacein viz., 2-(3,4-dihydroxyphenyl)ethanol, isoquercitrin and ursolic acid has been isolated from aerial parts has shown ACE inhibitory property(7).

  7. The leaves of Jasminum grandiflorum is used in folk medicine for treatment of ulcerative stomatitis, toothache, skin diseases, wounds and corns(8).

  8. The leaves extract is used as gargles (9).

  9. The ethanolic extract of Jasminum grandiflorum leaf has been reported as anti ulcer (10).

  10. Chemopreventive efficacy and anti-lipid peroxidative potential of Jasminum grandiflorum has been reported (11).

  11. Chameli shows spasmolytic activity(12).

7.



6.3 - Objective of the Study:


  • Collection of the authenticated plant material (i.e. leaves of Jasminum grandiflorum) from CCRAS, Bangalore.




  • Pharmacognostical studies of leaves.




  • Preparation of the extract of the drug.




  • Isolation of chemical constituents from the extract by column chromatography and fractionation method.




  • Identification of isolated constituents by IR and NMR spectroscopy.




  • Antimicrobial activity of the isolated compounds and extract.



Materials and Methods:

7.1 a) Source of Data:


Search on Medline and other Journals from The Oxford College of

Pharmacy, RGHUS-Digital library.

b) Place of work: The Oxford College of Pharmacy, Bangalore.

7.2 Method of Collection of Data:





  • Collection - Authenticated samples of leaves of Jasminum grandiflorum will be collected from CCRAS, Bangalore.

  • Pharmacognosy - T.S, stomatal index, vein islet no., palisade ratio and powder analysis for leaf sample.

  • Extraction - Extracts of the drugs will be prepared using different solvents by maceration/ soxhletion.

  • Isolation – Isolation of extracted phyto-constituents will be done using column chromatography/fractionation method.

  • Identification and characterization - The isolated compounds will be identified using analytical methods like IR and NMR spectroscopy.

  • Biological activity - The isolated compounds and extract will be studied for antimicrobial activity by agar diffusion method.

7.3 - Does the study require any investigations or inventions to be conducted on

patients or other humans or animals?

If so, please describe briefly.

- Not applicable -

7.4 - Has ethical clearance been obtained from your institution in case of 7.3?

- Not applicable -





8.


Bibliography:

  1. Nadkarni AK, Nadkarni KM. India Materia Medica, Vol-1, Popular Prakashan Pvt. Ltd, Bombay 1976; 701.

  2. The Wealth of India A Dictionary of Indian Raw Materials and Industrial Products, CSIR, New Delhi 2004; 284-88.

  3. Divakar NG, Subramanian V, Sugumaran M, Validyanathan CS. Indole oxygenase from the leaves of Jasminum grandiflorum. Plant Science Lette 1979; 15(2): 177-81.

  4. Sadhu SK, Khan MS, Ohtsuki T, Ishibashi M. Secoiridoid components from Jasminum grandiflorum. Phytochemistry 2007; 68(13):1718-21.

  5. Khare CP. India Medicinal Plants. An Illustrated Dictionary. Springer International Edition New Delhi 2007; 343-344.

  6. Cum G, Spadaro A, Citrara T, Gallo R. Processo di estrazionein fase supercritical da fioridi Jasminum grandiflorum L. Essenze Derivati Agrumari 1998; 68(4): 384-400.

  7. Somanadhan B, Smitt UW, George V, Pushpangadan P, Rajasekharan S, Duus JO, et al. Angiotensin converting enzyme(ACE) inhibitors from Jasminum azoricum and Jasminum grandiflorum. Planta Med 1998; 64(3):246-50.

  8. Stedman’s Medicinal Dictionary, 28th ed. Lippincott Williams & Wilkins, Philadelphia; 443.

  9. Khare CP. Encyclopedia of India Medicinal Plants, Rational Western Therapy, Ayurvedic and other traditional Usage. Botany, Springer-Verlag Berlin Heidelberg, New York 2007; 267-68.

  10. Umamaheswari M, Asokkumar K, Rathidevi R, Sivashanmugam AT, Subbhardadevi V, Ravi TK. Anti ulcer and in vitro antioxidant activities of Jasminum grandiflorum L. J Ethnopharmacol 2007; 110(3):464-70.

  11. Kolanjiappan K, Manoharan S. Chemopreventive efficacy and anti-lipid peroxidative potential of Jasminum grandiflorum Linn. On 7,12-dimethylbenz(a)anthracene-induced rat mammary carcinogenesis. Fundam Clin Pharmacol 2005; 19(6):687-93.

  12. Lis-Balchin M, Hart S, Wan Hang Lo B. Jasmine absolute (Jasminum grandiflora L.) and its mode of action on guinea-pig ileum in vitro. Phytother Res 2002; 16(5):437-9.




9.


Signature of Candidate




10.

Remarks of the Guide


Recommended




11.

11.1 Name and Designation of Guide




Dr. Padmaa M. Paarakh


Professor and Head

Department of Pharmacognosy







11.2 Signature








11.3 Co-Guide


_





11.4 Signature

_





11.5 Head of the Department

Dr. Padmaa M. Paarakh


Professor and Head

Department of Pharmacognosy






11.6 Signature


12.

12.1 Remarks of the Chairman and

Principal



Forwarded to the University for scrutiny





12.2 Signature

Dr. Padmaa M. Paarakh

Principal

The Oxford College of Pharmacy,



J.P.Nagar, Ist Phase, Bangalore – 78.



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