Induced by the Citrus psorosis virus




Дата канвертавання17.04.2016
Памер201.99 Kb.


Table S1. Symptomsz induced by the Citrus psorosis virus (CPsV) isolate PB-143 in different species, cultivar or hybrids of Citrus and related genera and relative accumulation of CPsV as detected by ELISA.





Symptoms in young leaves

Symptoms in fully expanded leaves







Species and hybrids

Shock

Chlorotic

Chlorotic

Chlorotic

Necrotic

Psorosis B

Pathogenicity

index


ELISA readingsx







flecking

blotching

blotching

etching




Indexy




Subtribe Citrinae:

























Citrus:

























C. myrtifolia large leaf

0

1

1

0

0

0

1.22

2.52 ± 1.08

C. macrophylla

1

0

2

1

1

0

6.40

2.75 ± 0.07

C. volkameriana

0

0

1

1

0

0

2.44

2.74 ± 0.08

C. excelsa

1

0

2

1

0

0

3.15 ± 0.34

3.15 ± 0.34

C. webberi

1

1

1

1

0

0

3.08 ± 0.222

3.08 ± 0.22

C. tachibana

1

1

2

1

0

0

3.30 ± 0.20

3.30 ± 0.20

C. sunki

1

2

2

3

0

1

2.94 ± 0.12

2.94 ±0.12

C. karna

1

0

2

2

0

0

2.69 ± 0.15

2.69 ± 0.15

C. macroptera

1

1

0

0

1

0

7.32

3.35 ± 0.02

C. hystrix

1

1

2

1

0

0

3.49 ± 0.11

3.49 ± 0.11

C. depressa

1

0

0

0

0

0

0.05 ± 0.20

0.05 ± 0.20

C. shunkokan

1

2

2

1

0

0

2.93 ± 0.57

2.93 ± 0.57

C. daoxianensis

1

2

1

1

0

0

4.27

3.02 ± 0.26

C. halimii

0

0

0

1

1

0

1.51 ± 1.66

1.51 ± 1.66

C. clementina

























var. Clemenules

0

1

3

2

0

1

5.79

3.56 ± 0.23

C. deliciosa

























var. Common mandarin

1

2

2

2

0

1

9.45

3.08 ± 0.08

C. reshni

























var. Cleopatra mandarin

0

1

1

1

0

0

1.83

0.03 ± 0.04

C. tangerina

























var. Parson's Special

0

1

2

1

0

0

3.05

1.60 ± 1.74

C. unshiu

























var. Clausellina Satsuma

0

2

1

1

0

0

1.83

3.27 ± 0.79

var. Owari Frost Satsuma

0

2

1

1

0

0

2.74

3.28 ± 0.48

C. sinensis

























var. Foyos navel

2

1

2

1

1

0

9.15

3.03 ± 1.30

var. Valencia late Frost

1

1

2

1

0

1

5.79

1.61 ± 1.60

C. aurantium

























var. Sevillano sour orange

0

1

2

1

0

0

4.88

0.04 ±0.19

C. grandis

























var. Deep Red pummelo

1

0

2

2

1

0

5.49

2.84 ± 0.34

C. paradisi

























var. Marsh grapefruit

0

2

2

0

0

1

4.27

2.46 ± 0.16

C. meyeri

























var. Meyer lemon

0

1

2

1

0

1

3.05

2.69 ± 0.98

C. jambhiri

























Rough lemon

1

2

2

2

0

1

7.32

1.93 ± 0.97

C. limon

























var. Fino 74 lemon

0

1

2

1

0

0

4.27

1.88 ± 0.95

C. limonia

























Rangpur lime

1

1

1

2

0

1

6.71

2.67 ± 0.07

C. medica

























var. 861-S-1 Etrog citron

0

1

1

2

0

0

4.27

2.11 ± 0.07

C. aurantifolia

























var. Mexican lime

1

1

2

1

0

0

3.35

2.99 ± 0.06

C. latifolia

























var. Bearss lime

1

0

3

1

0

0

6.10

3.42 ± 0.09

C. limettioides

























var. Palestine sweet lime

1

1

2

1

1

0

6.71

2.40 ± 0.10

C. junos

























Yuzu

0

0

1

1

0

0

2.13

0.60 ± 0.82

C. bergamia

























var. Calabria bergamote

0

0

1

1

0

0

4.27

1.41± 1.51

C. nobilis

























var. Campeona mandarin

1

1

1

1

0

0

3.96

3.28 ± 0.19

Poncirus:

























Poncirus trifoliata (Rubidoux)

n.e

n.e

n.e

n.e

n.e

n.e

n.e

n.e

Hybrids:

























Fortune mandarin (C. clementina

0

0

1

1

0

1

6.10

1.69 ± 1.75

x C. tangerina)

























Nova mandarin [C. clementina x

0

0

2

2

0

0

4.88

3.73 ± 0.28

(C. reticulata x C. paradisi)]

























Orlando tangelo [C. clementina

1

0

2

0

1

1

6.40

3.78 ±0.21

x (C. paradisi x C. tangerina)]

























Ortanique tangor (C. reticulata x C. sinensis)

1

1

2

1

1

0

10.98

1.72 ± 1.82

Carrizo citrange

2

0

0

0

0

0

5.49

-0.05± 0.04

(C. sinensis x P. trifoliata)

.'.






















Citrangequat 4 seasons [F. margarita x

0

1

1

0

0

0

0.91

3.56 ± 0.56

(C. sinensis x P. trifoliata)]

























CPB 4475 Citrumelo (C. paradisi x x P. trifoliata)

0

0

1

2

1

0

6.10

-0.02 ± 0.10

Microcitrus:

























M. inodora

0

0

0

0

0

0

0

2.81 ± 0.17

M. australis

1

1

1

2

0

0

6.10

3.30 ± 0.76

M. warburgiana

2

0

1

0

0

0

-

-

M. virgata

1

0

0

0

0

0

0.91

2.63 ± 0.47

M. sp.

0

0

0

0

0

1

1.83

4.21 ± 0.05

Fortunella:

























F. hindsii

0

0

0

0

0

0

0

3.60 ± 0.03

F. polyandra

0

0

1

0

0

0

0.61

1.18 ± 1.41

F. japonica

0

1

2

1

0

0

4.57

2.31 ± 1.42

F. crassifolia

0

1

1

1

·0

0

3.96

2.87 ± 0.64

F. obovata

1

0

1

1

0

1

3.96

3.48 ± 0.43

F. margarita

























Nagami kumquat

1

0

0

0

0

1

4.27

4.61 ± 0.19

Eremocitrus:

























Eremocitrus glauca

2

0

0

0

0

0

4.57

3.715 ± 0.29

Pleiospermium:

























Pleiospermium sp.

0

1

2

1

1

0

2.44

3.09 ± 0.57

Severinia:

























S. buxifolia

1

0

1

0

0

0

3.96

3.46 ± 0.49

Atalantia:

























A. ceylanica

O

1

2

1

1

O

2.44

2.85 ± 0.03

A. citroides

n.e

n.e

n.e

n.e

n.e

n.e

n.e

n.e

Subtribe Balsamocitrinae:

























Swinglea:

























S. glutinosa

n.e

n.e

n.e

n.e

n.e

n.e

n.e

n.e

Afraegle:

























A. paniculata

n.e

n.e

n.e

n.e

n.e

n.e

n.e

n.e

Subtribe Clauseninae:

























Clausena:

























Cl. excavata

1

O

O

O

O

O

0.91

0.04 ± 0.02


z Symptoms observed in most plants (other symptoms like necrotic etching, leaf deformation, secondary shocks or dwarfing were observed only in some plants). Intensity index for each symptom was evaluated from 0 to 3: 0= absence of symptoms; 1= mild symptoms in ≤25% of the leaves; 2= moderate symptoms in up to 50% of the leaves; 3= moderate to severe symptoms throughout the whole plant; n.e= not evaluated.

y The pathogenicity index was calculated using the formula: PI= [(3xS) + (FxI) + B + (2xBFxI) + (3xNExI) + (3xPB)]/2x100, where S is the number of plants showing shock reaction in the first flush, F is the number of plants with chlorotic flecking in young leaves, B, is the number of plants with chlorotic blotching in young leaves, BF is the number of plants with chlorotic blotching in fully expanded leaves, NE is the number of plants with necrotic etching in fully expanded leaves, PB is the number of plants with PsB lesions in old leaves, and I is the intensity index estimated for each symptom.

x Samples with ELISA readings more than three times the mean value of the healthy control were considered positive and used to calculate the mean values in the table. The mean negative control values for each plate were subtracted from each experimental ELISA value and from the mean positive control value. These adjusted experimental values were expressed as a ratio with the adjusted mean positive control value ± standard deviation.



Velázquez, Plant Disease




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