Identification of common horsetail Equisetum arvense




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Identification of common horsetail (Equisetum arvense L.; Equisetaceae) using Thin Layer Chromatography versus DNA barcoding- Saslis-Lagoudakis et al.

Supplementary Information



Supplementary Table 1. Details of materials used in the present study. Use in study: * DNA barcoding, + phylogeny of Equisetum, # TLC (E. arvense – E. palustre comparison), § TLC (all species of Equisetum).


Study ID

Species

Collector

Collection Number

Collection Date

Locality

Use in study

E. arvense 01

Equisetum arvense L.

M.B. Gerrans

1345

11-07-1967

Middlesex, UK

* + #

E. arvense 02

Equisetum arvense L.

G. Halliday

223/82

16-08-1982

Durham, UK

* + #

E. arvense 03

Equisetum arvense L.

C. Madsen

CM002

20-10-2013

Esrum Sø, Denmark

* + #

E. arvense 04

Equisetum arvense L.

C. Madsen

CM001

20-10-2013

Utterslev Mose, Denmark

* + #

E. arvense 05

Equisetum arvense L.

G. Williamson

106

05-07-1974

Pennsylvania, USA

* + #

E. arvense 06

Equisetum arvense L.

M. Blondeau

TQ92135

31-07-1992

Tasiujag, Canada

* + # §

E. arvense 08

Equisetum arvense L.

S.L. Jury

12030

25-06-1985

Reading, UK

* #

E. arvense 09

Equisetum arvense L.

B. Deylova

-

30-07-1976

Prague, Czech Republic

* #

E. arvense 10

Equisetum arvense L.

M.B. Gerrans

1345

11-07-1957

Middlesex, UK

* #

E. arvense 11

Equisetum arvense L.

H. Smith

1197

05-09-1921

China

* #

E. arvense 12

Equisetum arvense L.

V.B. Sochava & V.V. Lipatova

-

28-07-1956

Amur District, Russia

* #

E. arvense 13

Equisetum arvense L.

L.A. Gustavsson

9266

10-06-1985

Phocis, Greece

* #

E. arvense 14

Equisetum arvense L.

K. Hansen

6030

19-08-1960

Vågø, Faroe Islands

* #

E. arvense 15

Equisetum arvense L.

E. Pobedimova & F. Leisle

5101

14-08-1968

Leningrad, Russia

* #

E. arvense 16

Equisetum arvense L.

T. I. Alabina

-

18-09-1944

Ryazan District, , Russia

* #

E. arvense 17

Equisetum arvense L.

J.Ax. Nannfeldt

17940

13-07-1963

Gästrikland, Sweden

* #

E. arvense 18

Equisetum arvense L.

H.E. Ahles

86684

02-06-1953

Massachusetts, USA

* #

E. bogotense 01

Equisetum bogotense Kunth

A. & H. Adsersen

577

25-06-1974

Fernandina, Galapagos, Ecuador

+ §

E. diffussum 02

Equisetum diffusum D. Don

J.F. Rock

2730

02-03-1922

Southern Yunnan, China

+ §

E. fluviatile 01

Equisetum fluviatile L.

G.A. Matthews

-

21-06-1964

Hertfordshire, UK

+ §

E. fluviatile 02

Equisetum fluviatile L.

O. Skifte & M. Aasen

50886

17-09-1995

Troms, Norway

+

E. giganteum 01

Equisetum giganteum L.

A. Krapovickas & C.L Cristobal

17600

10-01-1971

Jujuy, Argentina

+ §

E. giganteum 02

Equisetum giganteum L.

G. Hatschbach

41942

18-01-1979

Curitiba, Brazil

+

Continued



















E. hyemale 01

Equisetum hyemale L.

T. Ulvinen & P. Hanhela

-

23-08-1984

Pudasjaervi, Finland

+ §

E. hyemale 02

Equisetum hyemale L.

T. Barta

1999-88

19-03-1999

Niederoesterreich, Austria

+

E. laevigatum 02

Equisetum laevigatum A. Braun

A. Nelson

7693

26-07-1900

Wyoming, USA

+ §

E. laevigatum 03

Equisetum laevigatum A. Braun

T. Ranker

EPOB 4520

10-06-1993

Colorado, USA

+

E. myriochaetum 01

Equisetum myriochaetum Schltdl. & Cham.

L. Holm-Nielsen & S. Jeppesen

280

03-06-1968

Tungurahua, Ecuador

+

E. myriochaetum 02

Equisetum myriochaetum Schltdl. & Cham.

L. Holm-Nielsen & S. Jeppesen

330

04-06-1968

Tungurahua, Ecuador

+ §

E. palustre 01

Equisetum palustre L.

G.A. Matthews

-

16-06-1961

North Devon, UK

* + #

E. palustre 02

Equisetum palustre L.

G.A. Matthews

-

24-05-1973

Durham, UK

* + #

E. palustre 03

Equisetum palustre L.

P. Paasovaara

-

06-07-1983

Kiiminki, Finland

* + #

E. palustre 04

Equisetum palustre L.

O. Seberg

460

15-08-1976

Gljufrafoss, Iceland

* + # §

E. palustre 05

Equisetum palustre L.

A. Strid et al.

44398

18-07-1997

Dramas, Greece

* + #

E. palustre 06

Equisetum palustre L.

G. Pettersen

C2944

21-05-2013

Emilia - Romagna, Italy

* + #

E. palustre 08

Equisetum palustre L.

K. Hansen

1607

27-07-1960

Fjallmannaheyggjur, Faroe Islands

* #

E. palustre 09

Equisetum palustre L.

K. Hansen

2136

03-08-1960

Strømø, Faroe Islands

* #

E. palustre 10

Equisetum palustre L.

Strid & Mikkelsen

34887

05-05-1993

Arkadias, Greece

* #

E. palustre 11

Equisetum palustre L.

V.V. Mararov

-

20-06-1970

Solnetschno-gorsk, Russia

* #

E. palustre 12

Equisetum palustre L.

P. Monserrat

3182/71

18-06-1971

Jaca, Spain

* #

E. palustre 13

Equisetum palustre L.

L. Edelberg

1328

13-07-1948

Nuristan, Afghanistan

* #

E. palustre 14

Equisetum palustre L.

Jon Kaasa

-

21-08-1971

Oppland, Norway

* #

E. palustre 15

Equisetum palustre L.

P. Hartvig, C. Baden & S.G. Christiansen

6081b

02-07-1976

Epirus, Greece

*

E. palustre 16

Equisetum palustre L.

L.A. Gustavsson

9446

07-12-1985

Phocis, Greece

* + #

E. palustre 17

Equisetum palustre L.

N. Maltzev

7304a

20-07-1905

Irkutsk, Russia

* + #

E. palustre 18

Equisetum palustre L.

W.J. Cody

26663

02-07-1980

Yukon, Canada

#

E. pratense 02

Equisetum pratense Ehrh.

G. Shaughnessy

78

24-06-1992

Alaska, USA

+ §

E. ramosissimum 01

Equisetum ramosissimum Desf.

M.R. Parishani

14311

23-05-2003

Isfahan, Iran

+

E. ramosissimum 02

Equisetum ramosissimum Desf.

M.G. Gilbert et al.

7334

01-05-1983

Shewa, Ethiopia

+ §

Continued



















E. ramosissimum 03

Equisetum ramosissimum Desf.

H.H. Bruun

43

28-02-1990

Gomera, Canary islands, Spain

+

E. scirpoides 01

Equisetum scirpoides Michx.

M. Blondeau

M-92087

12-06-1992

Quebec, Canada

+ §

E. sylvaticum 01

Equisetum sylvaticum L.

K. Nisula & P. Renvall

-

03-08-1999

Kuopio, Finland

+ §

E. telmateia 01

Equisetum telmateia Ehrh.

E. Bermeier & U. Matthaes

4113

28-07-1994

Crete, Greece

+

E. telmateia 03

Equisetum telmateia Ehrh.

L. & V. Dalgaard

13246

04-09-1983

Madeira, Portugal

+ §

E. telmateia 04

Equisetum telmateia Ehrh.

J.S. Andersen & A.G. Jensen

7101

19-06-1973

Sari, Iran

+

E. variegatum 01

Equisetum variegatum Schleich. ex F. Weber & D. Mohr

T. Alm & J. Reiersen

-

31-08-1990

Troms, Norway

+

E. variegatum 02

Equisetum variegatum Schleich. ex F. Weber & D. Mohr

P.F. Zika

1306

14-05-1980

Massachusetts, USA

+

E. variegatum 04

Equisetum variegatum Schleich. ex F. Weber & D. Mohr

T. Neczaeva

-

15-08-1968

Sakhalin, Russia

* + §

Supplementary Table 2. Details of herbal products used in the present study.



Study ID

Form

E. arvense mentioned on package

A - USA

Tea

Y

B - Bulgaria

Tea

Y

F - Germany

Tea

N

MF - UK

Capsules

Y

NA - USA

Capsules

Y

NW - USA

Capsules

Y

HB - UK

Capsules

N

I - UK

Capsules

Y

Supplementary Table 3. Genbank accession numbers for all material used in the phylogenetic and DNA barcoding analyses.



Study ID

Genbank accession number

 

rbcL

matK

trnH-psbA

rsp4

ITS2

E. arvense 01

TBA

TBA

TBA

TBA

-

E. arvense 02

-

TBA

TBA

TBA

TBA

E. arvense 03

-

TBA

TBA

TBA

-

E. arvense 04

TBA

TBA

TBA

TBA

-

E. arvense 05

-

TBA

TBA

TBA

TBA

E. arvense 06

TBA

TBA

TBA

TBA

TBA

E. arvense 07

AY226140

-

-

AJ583677

-

E. arvense 08

-

TBA

TBA

-

-

E. arvense 09

-

-

TBA

-

-

E. arvense 10

-

TBA

TBA

-

-

E. arvense 11

-

TBA

TBA

-

-

E. arvense 12

-

TBA

TBA

-

-

E. arvense 13

-

-

TBA

-

-

E. arvense 14

-

TBA

TBA

-

-

E. arvense 15

-

TBA

TBA

-

-

E. arvense 16

-

TBA

-

-

-

E. arvense 17

-

-

TBA

-

-

E. arvense 18

-

TBA

TBA

-

-

E. bogotense 01

TBA

-

TBA

TBA

TBA

E. bogotense 02

AY226139

-

-

AJ583678

-

E. diffussum 01

AY226141

-

-

AJ583679

-

E. diffussum 02

-

TBA

TBA

-

-

E. fluviatile 01

TBA

TBA

TBA

TBA

-

E. fluviatile 02

TBA

TBA

-

TBA

TBA

Supplementary Table 3 Continued
















E. fluviatile 03

AY226142

-

-

AJ583680

-

E. giganteum 01

-

TBA

-

TBA

-

E. giganteum 02

TBA

TBA

TBA

-

TBA

E. giganteum 03

AY226127

-

-

AJ583681

-

E. hyemale 01

TBA

TBA

-

TBA

-

E. hyemale 02

-

TBA

-

TBA

-

E. hyemale 03

AY226128

-

-

AJ583682

-

E. laevigatum 01

AY226130

-

-

AJ583683

-

E. laevigatum 02

TBA

TBA

TBA

TBA

TBA

E. laevigatum 03

-

TBA

TBA

TBA

TBA

E. myriochaetum 01

TBA

-

-

TBA

TBA

E. myriochaetum 02

TBA

-

TBA

TBA

TBA

E. myriochaetum 03

AY226131

-

-

AJ583684

-

E. palustre 01

TBA

-

TBA

TBA

TBA

E. palustre 02

-

-

TBA

TBA

TBA

E. palustre 03

TBA

TBA

TBA

TBA

TBA

E. palustre 04

-

-

TBA

TBA

TBA

E. palustre 05

TBA

TBA

TBA

TBA

TBA

E. palustre 06

TBA

TBA

TBA

TBA

-

E. palustre 07

AY226138

-

-

AJ583685

-

E. palustre 08

-

-

TBA

-

-

E. palustre 09

-

-

TBA

-

-

E. palustre 10

-

TBA

TBA

-

-

E. palustre 11

-

TBA

-

-

-

E. palustre 12

-

TBA

TBA

-

-

E. palustre 13

-

TBA

-

-

-

E. palustre 14

-

TBA

TBA

-

-

Supplementary Table 3 Continued
















E. palustre 15

-

TBA

TBA

-

-

E. palustre 16

-

-

TBA

-

-

E. palustre 17

-

-

TBA

-

-

E. pratense 01

AY226137

-

-

AJ583686

-

E. pratense 02

TBA

-

TBA

TBA

-

E. ramosissimum 01

TBA

TBA

TBA

TBA

TBA

E. ramosissimum 02

TBA

TBA

-

TBA

TBA

E. ramosissimum 03

TBA

-

-

TBA

TBA

E. ramosissimum 04

AY226132

-

-

AJ583687

-

E. scirpoides 01

-

TBA

TBA

TBA

-

E. scirpoides 02

AY226133

-

-

AJ583688

-

E. sylvaticum 01

-

TBA

TBA

TBA

TBA

E. sylvaticum 02

AY226136

-

-

AJ583689

-

E. telmateia 01

TBA

-

TBA

TBA

-

E. telmateia 02

AY226135

-

-

AJ583690

-

E. telmateia 03

-

TBA

TBA

TBA

TBA

E. telmateia 04

-

-

TBA

TBA

TBA

E. variegatum 01

-

TBA

TBA

TBA

-

E. variegatum 02

-

TBA

TBA

TBA

-

E. variegatum 03

AY226134

-

-

AJ583691

-

E. variegatum 04

TBA

TBA

TBA

TBA

-

Herbal product B - Bulgaria

-

TBA

-

-

-

Herbal product F - Germany

-

TBA

TBA

-

-

Angiopteris angustifolia

NC_026300

-

-

-

-

Ophioglossum reticulatum

AY138410

-

-

AF313594

-





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