P. O. Box 65175/4161, Hamedan, Iran *e-mail: R_karamian@basu.ac.ir
Received November 6, 2007; revision accepted May 13, 2008
An efficient procedure is established here for plant regeneration from hypocotyl explants in Onobrychis subnitens Bornm., an important forage legume in Iran. Two types of calli, embryogenic and non-embryogenic, wereinduced from hypocotyl explants on MS medium supplemented with 2,4-D and BAP at different concentrations.To initiate morphogenesis, embryogenic calli were transferred to MS medium with 0.5 mg·l-1 NAA and 1 mg·l-1BAP. Initiated somatic embryos or adventitious buds developed into plantlets following culture on MS medium without any growth regulators or with 2 mg·l-1 NAA, respectively. All the regenerated plants were normal with respect to morphology and growth characters.
Key words: Hypocotyl culture, Onobrychis subnitens Bornm., organogenesis, plant regeneration, somatic embryogenesis.