Controlled crosses (female x male)




Дата канвертавання18.04.2016
Памер71.75 Kb.




Parent genotypes

Offspring
















Controlled crosses

(female x male)



Marker













Genotype

Observed number (O)

Expected Mendelian segregation

Expected number (E)

* P value

Cs79 x Cs18

26 seedlings



cyp52

174/174 x 174/174

174/174

26

---

---

---

cyp84

242/248 x 248/248

242/248

248/248


15

11


1

1


13

13


0.56

cyp101

182/184 x 184/184

182/184

184/184


18

8


1

1


13

13


0.08

cyp174

208/214 x 210/214

208/210

208/214


210/214

214/214


9

4

7



6

1

1

1



1

6.5

6.5


6.5

6.5


0.59

cyp257

163/183 x 163/null

163/163, 163/null

163/183


183/null

15

3

8



2

1

1



13

6.5


6.5

0.36

cyp258

226/226 x 226/230

226/226

226/230


15

11


1

1


13

13


0.56

cyp293

185/185 x 185/185

185/185

26

---

---

---

Cs129 x Cs32

13 seedlings

Cs32 x Cs129

12 seedlings



cyp52

174/174 x 174/186

174/174

174/186


15

10


1

1


12.5

12.5


0.43

cyp84

244/248 x 248/null

244/null

244/248


248/248, 248/null

5

4

16



1

1

2



6.25

6.25


12.5

0.40

cyp101

182/184 x 184/null

182/null

182/184


184/184, 184/null

4

8

13



1

1

2



6.25

6.25


12.5

0.55

cyp174

208/208 x 214/214

208/214

25

---

---

---

cyp257

159/163 x 163/163

159/163

163/163


12

12


1

1


12

12


1.00

cyp258

226/234 x 230/242

226/230

226/242


230/234

234/242


7

6

8



4

1

1

1



1

6.25

6.25


6.25

6.25


0.76

cyp293

185/185 x 185/185

185/185

25

---

---

---

Cs48 x Cs62

13 seedlings

Cs62 x Cs48

13 seedlings



cyp52

174/174 x 174/186

174/174

174/186


13

13


1

1


13

13


1.00

cyp84

242/248 x 242/248

242/242

242/248


248/248

5

14

7



1

2

1



6.5

13

6.5



0.87

cyp101

182/184 x 184/184

182/184

184/184


12

14


1

1


13

13


0.84

cyp174

208/208 x 208/214

208/208

208/214


15

11


1

1


13

13


0.56

cyp257

163/183 x 163/null

163/163, 163/null

163/183


183/null

12

6

8



2

1

1



13

6.5


6.5

0.88

cyp258

226/226 x 226/230

226/226

226/230


17

9


1

1


13

13


0.17

cyp293

185/185 x 185/185

185/185

26

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Table 2. SSR marker inheritance and allele segregations among 77 Cupressus sempervirens seedlings produced by controlled crosses.

*Probability of the observed segregation under Mendelian inheritance hypothesis, computed from the distribution of simulated segregations over 1000 Monte Carlo replicates







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