Biotransformation of menthol and geraniol by hairy root cultures of Anethum graveolens: effect on growth and volatile components




Дата канвертавання22.04.2016
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Biotransformation of menthol and geraniol by hairy root cultures of Anethum graveolens: effect on growth and volatile components

Jorge M.S. Faria, Inês S. Nunes, A. Cristina Figueiredo, Luis G. Pedro, Helena Trindade, José G. Barroso


Universidade de Lisboa, Faculdade de Ciências de Lisboa, Departamento de Biologia Vegetal, Instituto de Biotecnologia e Bioengenharia, Centro de Biotecnologia Vegetal, C2, Campo Grande, 1749-016 Lisbon, Portugal
Section: Plant Cell Technology
Supplementary Table 1 – Percentage composition of the substrates (menthol or geraniol) and the corresponding biotransformation products isolated from Anethum graveolens hairy root cultures maintained for 6 weeks in SH medium, in darkness at 24ºC and 80r.p.m..

Components

RI

Substrate added Anethum graveolens hairy root cultures

Time after addition (hours)

1

4

8

24

32

48

168

336

504

672

Menthol

1148

51.3

40.5

45.8

28.3

19.0

10.7

11.2

3.8

3.3

5.3

Menthyl acetate

1278

3.8

8.0

11.6

35.4

45.8

54.6

43.1

39.4

28.1

21.3





































Geraniol

1236

20.3

15.2

10.9

7.3

3.7

2.8

2.2

0.9

0.7

1.7

Linalool

1074

1.7

2.0

2.2

2.4

4.7

3.2

3.2

1.0

1.0

1.1

α-Terpineol

1159

0.5

1.0

1.3

1.5

2.2

1.6

2.8

1.0

0.6

0.6

Citronellol

1210

5.2

6.1

6.7

6.1

2.6

1.6

2.3

0.9

0.8

0.6

Neral

1210

2.0

2.3

3.6

2.5

0.5

0.2

1.0

0.7

0.4

0.1

Geranial

1240

3.4

2.7

3.9

2.6

0.5

0.2

0.2

t

t

0.1

Citronellyl acetate

1343

0.2

0.5

0.7

3.4

4.3

4.7

5.9

4.3

3.3

1.4

Neryl acetate*

1275

2.6

5.8

7.1

16.8

17.6

18.5

16.5

6.8

2.9

0.6

Geranyl acetate

1370

7.6

12.5

12.4

16.7

22.4

23.5

10.4

3.0

0.5

0.6

RI: Retention index relative to C9-C22 n-alkanes on the DB-1 column; t: traces (<0.1%). * Identification based on mass spectra only.

Supplementary Fig. 1. Biosynthetic scheme showing the probable relationship between substrates (menthol or geraniol) and the corresponding biotransformation products isolated from Anethum graveolens hairy root cultures.




Corresponding author: Ana Cristina Figueiredo, Phone number: +351217500257, Fax: +351217500048, E-mail address: acsf@fc.ul.pt



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