Annexure – II




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RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

BANGALORE, KARNATAKA

ANNEXURE – II



PROFORMA FOR REGISTRATION OF SUBJECT DISSERTATION

1

NAME OF THE CANDIDATE AND ADDRESS




Mr. PURI ABHIJEET VISHNU


Department of Pharmacognosy & Phytochemistry,

K.L.E.Society’s College of Pharmacy,

Vidyanagar, HUBLI- 580031

2

NAME OF INSTITUTION

K.L.E.SOCIETY’S COLLEGE OF PHARMACY,

VIDYANAGAR, HUBLI-580031

3

COURSE OF STUDY AND SUBJECT

MASTER OF PHARMACY

IN

PHARMACOGNOSY

4

DATE OF ADMISSION OF COURSE

JUNE-2008



5

TITLE OF THE TOPIC:
PHYTOCHEMICAL INVESTIGATIONS AND EVALUATION OF

Diospyros Montana Roxb. LEAVES FOR ANTIOXIDANT ACTIVITY.





6.




BRIEF RESUME OF THE INTENDED WORK:

6.1 Need for the study :

An Antioxidant is a molecule capable of slowing or preventing oxidation of other molecules. Antioxidants are believed to quench free radicals. Free radicals are atoms or molecules with ‘singlet’, i.e. unpaired electron which makes them highly reactive. Oxidative free radicals are generated by metabolic reactions, create a chain reaction leading to membrane and other lipid peroxidation, DNA damage etc. This has been implicated in atherosclerosis (Oxidized LDL is more atherogenic) cancers, neurodegenerative diseases and inflammatory bowel diseases. Many endogenous and dietary compounds like super oxide dismutase, ferritin, transferrin, ceruloplasmin,  Tocopherol,  carotene and ascorbic acid have antioxidant and free radical scavenging properties. 1

Traditionally plants such as Allicin, Amla, and Turmeric are used to treat various inflammatory diseases like Cancer, atherosclerosis, premature aging, immuno-stimulant properties, as well as anti-inflammatory in which free radical oxidative stress has been implicated in the pathogenesis of these disorders. 2

Ayurveda and various traditional system of medicine claim that Diospyros Montana



Roxb has been used in the treatment of various diseases like delirium high fever

,jaundice, diarrhea ,tuberculosis and cancer and leishmaniasis.4 And the plant contains

Diospyrin , β- sitosterol, α –amyrin , β- amyrin, Lupeol , Taraxerol, Stigmasterol,

Betulin Oleanolic acid Betulinic and, Kaempferol . 5

Studies showed that several natural products possessing antidiabetic,antitumor and also

flavonoids containing drugs can act as antioxidant. Till now no scientific papers are

available on the leaves of Diospyros Montana Roxb for antioxidant activity. Hence this

research work has been taken up.







6.2 Review of literature :
Introduction to the Plant:

Botanical Name : Diospyros Montana Roxb.

Family : Ebenaceae

Vernacular names:

Kannada: Balagani.

Marathi: Thembhurni.

Gujrati : Timbaroa

Tamil: Karunthuvalism.

Sanskrit: Kaaka thinduka 6
Morphology:

Diospyros Montana Roxb is a small tree with slender stem and smooth grey bark

young shoots glabrous or pubescent. Leaves alternate , ovate-oblong or elliptical acute or

sub acuminate, base usually rounded , softly pubescent or tomentose when young ultimately

glabrescent. 7



Phytoconstituents:

Plant contains the following Phytoconstituents .Kaempferol diglucoside , Quercetine ,

Gallic acid , Leupeol, Betulin , Betulinic acid , Ursolic acid, α-amyrin, β-amyrin ,Oleanolic

acid , β-Sitosterol, Sigmasterol Diospyrin, β- Dihydrodiospyrin , 8-Hydrodiospyrin,

Chromenone acid. 5

Traditional Uses:

The plant is used in indigenous medicine for delirium high fevers and to treat vomiting,

Jaundice, diarrhea, and pneumonia. It is an antibacterial and effective against organisms like

Bacillus subtilis. It also shows CNSdepressent, spasmolytic and anticancer agent activity. 8


1 N. Lall1, M. Das Sarma2, B. Hazra and J. J. M. Meyer1 (2003)

Antimycobacterial activity of diospyrin derivatives and a structural analogue of diospyrin against

Mycobacterium tuberculosis in vitro

Diospyrin was isolated from the stem-bark of D. Montana Roxb The activity of diospyrin against

drug susceptible and -resistant strains of M. tuberculosis has been reported .Three derivatives

and one structural analogue of diospyrin were synthesized and investigated

for their inhibitory activity against Mycobacterium tuberculosis employing the rapid radiometric

method in vitro 9











Reported Works:-
B.N. Shina et.al Reviewed the Phytochemical and Biological Studies of Diospyros Species

used in folklore medicine of Jharkhand.5

N. Lall, M. Das Sarma et.al Isolated Diospyrin from the stem-bark of D. Montana Roxb and

reported the activity of diospyrin against drug susceptible resistant strains of M. tuberculosis.

Three derivatives and one structural analogue of diospyrin were synthesized and investigated

for their inhibitory activity against Mycobacterium tuberculosis employing the rapid

radiometric methodin vitro. 9

B. Hazra, J. Golenser et.al Isolated Diospyrin from the stem bark of Diospyros Montana Roxb.

Synthesized its derivatives and reported the inhibitory activity against Leishmania major

Promastigotes.10

M. Maridass Isolated and characterized various Phytochemical from Diospyros species.

These include Napthaquinones, triterpenoid and steroid. Notable activities are reported

from the various part of the plants . Also the constituents for antibacterial , antifungal,

antiprotozoal, antimolluscocidal , anti-inflammatory and cytotoxic activity was isolated.11








7




6.3 The objectives of the present study are:



  • Pharmacognostic Evaluation of Diospyros Montana Roxb. Leaves.

  • Extraction and phytochemical analysis.

  • Phytochemical investigation of various extracts of Diospyros Montana Roxb. Leaves.

  • In- vitro evaluation of the extracts for antioxidant activity.

  • Isolation and characterization of phytochemical constituents from extracts having maximum activity using column chromatography and Spectral studies respectively.


MATERIALS AND METHODS:
7.1 Source of data


  • Published research papers.




  • Review articles from Journals.




  • Electronic data.



  • Library of K.L.E.S’s College of pharmacy.



7.2 Method of collection:


Collection: - The plant Diospyros Montana Roxb. will be collected from the local

Surrounding areas of Hubli, Mundagod and Katur.


Authentication: - Renowned botanist will authenticate the plant.
Pharmacognostic studies:- Pharmacognostic evaluation like morphology,

macroscopic and microscopic. Evaluation will be carried out on



Diospyros Montana Roxb leaves.
Phytochemical studies: - For the present study leaves of Diospyros Montana Roxb.

will be collected shade dried and coarsely powdered .Extraction

will be done by using different solvents of increasing polarity using

soxhlet apparatus.








In vitro antioxidant activity:- In vitro antioxidant activity will be carried on the

extracts using DPPH free radical scavenging assay, Nitric oxide

Radical scavenging assays 12 etc.

Isolation and characterization :- The phytoconstituents from extracts having

maximum activity will be isolated and characterized by using

column- chromatography and spectral analysis respectively.





7.3 Does the study require any investigation or interventions to be conducted on the

ssspatients or other human/animals? If so, please describe briefly.

-----------------------------NO------------------------------

7.4 Has ethical clearance been obtained from your institution in case of 7.3?
--------------------NOT APPLICABLE--------------------







8.



LIST OF REFERENCES:
1. Tripathi K D Essentials of Medicals Pharmacology. 5th ed. New Delhi: Jaypee Brothers Medical Publishers (P) Ltd; 2003.
2. Naik S R. Antioxidant and their role in biological functions: An overview, Indian Drugs.

2003 September;40:9.


3 Yoganarasimhan SN. Medicinal Plants of India., Bangalore, Interline Publishing Pvt. Ltd.;

Vol.-I 1996.


4 Mallavadhani U. V, Panda. A. K, Rao .Y. R. Pharmacology and Chemotaxonomy of

Diospyros Phytochemistry 1998; 49: 901-51.


5 Shina B.N, Bansal.S.K. A review of Phytochemical and Biological Studies of Diospyros

Species used in folklore medicine of Jharkhand. Journal of Natural Remedies 2008; 8(1): 11-7.

6 Dr Magdi, R. Gurudev Msc PhD. Botanical and Vernacular names of South India plants ,

Divyachandara Prakashan , Bangalore : 164


7 Kirtikar KR, Basu BD. Indian Medicinal Plants India, vol-II. International book distribution 1987.
8 Anonymous. The Wealth of India. A Dictionary of Indian Raw Materials and Industrial

Products Publication & Information Directorate, Vol-3 (A), New Delhi: CSIR;

reprint: 2003.

9 Lall1N, Sharma M. Das, Hazra B, Meyer J. J. M. Antimycobacterial activity of diospyrin

derivatives and a structural analogue of Diospyrin against Mycobacterium tuberculosis in vitro.

J. Antimic. Chemo (2003); 51: 435–8.

10 Hazra. B, Golenser .J . et.al Inhibitory activity of Diospyrin derivatives against Leishmania

Major parasite in Vitro Ind J. pharmacol 2002 ; 34: 422-7.

11 Maridass. M. Phytochemicals From Genus Diospyros (L.) and their Biological Activities

Ethno botanical Leaflets 2008; 12: 231-44.

12 Jain P.K. and Agrawal. R.K. Antioxidant and Free Radical Scavenging Properties of

Developed Mono- and Polyhedral Formulations. Asian J. Exp. Sci 2008; 22, ( 3): 213-20









































































9.


SIGNATURE OF THE CANDIDATE


10.


REMARKS OF THE GUIDE


The work, which is assigned to Mr. PURI ABHIJEET VISHNU will be Carried out under my supervision and guidance.



11.


11.1 NAME AND DESIGNATION

OF THE GUIDE




Mrs. Kusum .S. AKKI


Senior Lecturer

Department of Pharmacognosy & Phytochemistry

K.L.E.S’s College of Pharmacy,

Vidyanagar, Hubli-31





11.2 SIGNATURE







11.3 NAME OF THE CO-GUIDE



----------------------





11.4 SIGNATURE


---------------------


12.

12.1 HEAD OF THE

DEPARTMENT



Mr. R. V. Savadi B. Sc, M.Pharm

Associate Professor,

Head of Department


Department of Pharmacognosy& Phytochemistry

K.L.E.S’s College of Pharmacy,

Vidyanagar,

Hubli – 580 031.






12.2 SIGNATURE



13.


13.1 REMARKS OF THE PRINCIPAL




The above-mentioned information is correct and I recommend the same for approval.





13.2 SIGNATURE







Dr. B.M.PATIL M Pharm. PhD.

Principal,

K.L.E.’S College of Pharmacy,

Vidyanagar, Hubli – 580 031.





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