[1]红外动目标识别跟踪系统的dsp+fpga实现




старонка15/19
Дата канвертавання24.04.2016
Памер0.94 Mb.
1   ...   11   12   13   14   15   16   17   18   19

Ultraviolet Imaging \Detection


302

Author(s): Schmolke, M (Schmolke, Marion); Fabian, J (Fabian, Joerg); Lehr, M (Lehr, Matthias)

Title: High-performance liquid chromatographic assay with ultraviolet spectrometric detection for the evaluation of inhibitors of phosphatidylinositol-specific phospholipase C

Source: ANALYTICAL BIOCHEMISTRY, 375 (2): 291-298 APR 15 2008

Abstract:

A nonradioactive spectrometric assay for the evaluation of inhibitors of phosphatidylinositol-specific phospholipase C (PI-PLC) is described. L-alpha-Phosphatidylinositol from bovine liver was used as substrate in the presence of the micelle-forming detergent deoxycholic acid. PI-PLC isolated from Bacillus cereus and crude cytosol fractions from porcine brain were used as enzyme sources. PI-PLC activity was determined by measuring the release of 1-stearoyl-2-arachidonoyl-sn-glycerol with reversed-phase HPLC and UV detection at 200nm. PI-PLC from B. cereus was not inhibited by the putative PI-PLC inhibitors U-73122 and ET- 18-OCH3 at 100 mu M, whereas the isobenzofuranone derivative 5 blocked the enzyme with an IC50 of 75 mu M. PI-PLC activity present in porcine brain cytosol was decreased by all three test compounds at 100 mu M to approximately 30 to 50%. (c) 2007 Elsevier Inc. All rights reserved.

Addresses: Univ Munster, Inst Pharmaceut & Med Chem, D-48149 Munster, Germany

Reprint Address: LEHR, M, UNIV MUNSTER, INST PHARMACEUT & MED CHEM, D-48149 MUNSTER, GERMANY

Author's E-mail: lehrm@uni-muenster.de

--------------------------------------------------------------------------------

303

Author(s): Pereira, AS (Pereira, Alberto S.); Schelfaut, M (Schelfaut, Marc); Lynen, F (Lynen, Frederic); Sandra, P (Sandra, Pat)



Title: Design and evaluation of a multi-detection system composed of ultraviolet, evaporative light scattering and inductively coupled plasma mass spectrometry detection for the analysis of pharmaceuticals by liquid chromatography

Source: JOURNAL OF CHROMATOGRAPHY A, 1185 (1): 78-84 MAR 21 2008

Abstract:

Reversed-phase liquid chromatography was coupled to a multi-detection system composed of ultraviolet (UV) detection, evaporative laser scattering detection (ELSD) and inductively coupled plasma mass spectrometry (ICP-MS). By applying the principle of post-column solvent compensation, the organic modifier content was kept constant in ELSD and ICP-MS under gradient elution. Chlorine (Cl-35), bromine (Br-79 and Br-81) and sulfur (S-34) were monitored in several pharmaceutical compounds. The limit of quantitation (LOQ) was 80 ng/rnL for chlorine (chlorpropamide) and 2 ng/mL for bromine (bromazepam). Calibration graphs were linear from 1.0 mu g/mL to 100 mu g/mL for chlorpropamide (r(2) 0.990) and from 10 ng/mL to 500 ng/mL for bromazeparn (r(2) 0.996). The low LOQ value for bromine allows to quantify bromine in pharmaceutical samples below the 0.05% level of the active pharmaceutical ingredient. (C) 2008 Elsevier B.V. All rights reserved.

Addresses: Univ Ghent, PARC, Dept Organ Chem, B-9000 Ghent, Belgium

Reprint Address: SANDRA, P, UNIV GHENT, PARC, DEPT ORGAN CHEM, KRIJGSLAAN 281 S4-BIS, B-9000 GHENT, BELGIUM

Author's E-mail: pat.sandra@richrom.com

--------------------------------------------------------------------------------

304

Author(s): Myakishev, M (Myakishev, Max); Polesskaya, O (Polesskaya, Oksana); Kulichkova, V (Kulichkova, Valentina); Baranova, A (Baranova, Ancha); Gause, L (Gause, Larissa); Konstantinova, I (Konstantinova, Irina)



Title: PCR-based detection of Pol III-transcribed transposons and its application to the rodent model of ultraviolet response

Source: CELL STRESS & CHAPERONES, 13 (1): 111-116 MAR 2008

Abstract:

Cellular levels of RNAs containing transposable elements increase in response to various stresses. Polymerase III (Pol III)-dependent transcripts of transposons are different from transposon-containing RNAs generated by read-through Pol II-dependent transcription. Until now, Pol III transcripts were detected by primer extension followed by time-consuming gel electrophoresis. In this paper, we describe a more sensitive PCR-based method for the selective detection of Pol III-transcribed RNAs. The method is based on the difference in sequences at the 5' ends of the Pol II- and Pol III-dependent transcripts. We employed this method to quantify Pol III transcripts of transposon B1 in rodent cells and revealed that their levels are affected by UV irradiation. We therefore conclude that the abundance of the Pol III-transcribed fraction of cellular RNA may serve as marker of stress response and can be conveniently quantified by the method described.

Addresses: Univ Rochester, Med Ctr, Dept Dermatol, Rochester, NY 14642 USA

Univ Rochester, Med Ctr, Dept Microbiol & Immunol, Rochester, NY 14642 USA

Russian Acad Sci, Inst Cytol, Lab Regulat Gene Express, St Petersburg 194064, Russia

George Mason Univ, Mol & Microbiol Dept, Manassas, VA USA

Russian Acad Med Sci, Med Genet Res Ctr, Moscow, Russia

An Koltsov Inst Dev Biol, Genet Lab, Moscow 117334, Russia

Reprint Address: MYAKISHEV, M, UNIV ROCHESTER, MED CTR, DEPT DERMATOL, POB 697, ROCHESTER, NY 14642 USA

Author's E-mail: Max_Myakishev@urmc.rochester.edu

--------------------------------------------------------------------------------

305


Author(s): Yoshikawa, M (Yoshikawa, M.); Murakami, M (Murakami, M.); Ishida, H (Ishida, H.)

Title: Highly sensitive detection of near-field Raman scattered light from strained Si/SiGe heterostructures by scanning near-field optical Raman microscope using ultraviolet resonant Raman scattering - art. no. 091903

Source: APPLIED PHYSICS LETTERS, 92 (9): 91903-91903 MAR 3 2008

Abstract:

We have developed a scanning near-field Raman microscope (SNORM) with a hollow pyramidal probe that uses ultraviolet resonant Raman scattering and measured changes in the near-field and far-field Raman intensities of strained Si/SiGe heterostructures as a function of a probe-sample distance R. We observed that the near-field Raman intensity of a strained Si film dramatically decreased with an increase in the probe-sample distance. The decay curve of the near-field Raman intensity was approximately expressed by the R-3 plot calculated from a simple model based on dipole-dipole interaction. This confirms that our SNORM detects the near-field light from the sample.

Addresses: Toray Res Ctr Ltd, Shiga 5208567, Japan

Reprint Address: YOSHIKAWA, M, TORAY RES CTR LTD, SONOYAMA 3-3-7, SHIGA 5208567, JAPAN

Author's E-mail: masanobu_yoshikawa@trc.toray.co.jp

--------------------------------------------------------------------------------

306


Author(s): Bedor, DCG (Bedor, D. C. G.); Goncalves, TM (Goncalves, T. M.); Ferreiraa, MLL (Ferreiraa, M. L. L.); de Sousaa, CEM (de Sousaa, C. E. M.); Menezes, AL (Menezes, A. L.); Oliveira, EJ (Oliveira, E. J.); de Santana, DP (de Santana, D. P.)

Title: Simultaneous determination of sulfamethoxazole and trimethoprim in biological fluids for high-throughput analysis: Comparison of HPLC with ultraviolet and tandem mass spectrometric detection

Source: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 863 (1): 46-54 FEB 15 2008

Abstract:

The comparison of two methods based on online solid phase extraction-liquid chromatography with UV (SPE-LC-UV) or mass spectrometry detection (SPE-LC-MS/MS) for the simultaneous quantification of sulfamethoxazole (SMZ) and trimethoprim (TMP) is presented. The methods were validated and proved to be accurate. The analysis of standard samples for SMZ at concentrations of 0.5, 1.5, 25 and 50 mu g/mL demonstrated a relative standard deviation of less than 6% for both methods (n = 18), while TMP samples at concentrations of 0.05, 0.15, 1.5 and 5.0 mu g/mL were analyzed with R.S.D. of less than 4% (n = 18). The method with mass spectrometric detection was approximately six times more sensitive than the method with ultraviolet detection. The total run time for the SPE-LC-MS/MS was 2.5 min per sample as opposed to 18.0 min for the SPE-LC-UV method. The method with MS detection in comparison with UV detection proved to be more rugged and was successfully applied to pharmacokinetics studies. (C) 2008 Elsevier B.V. All rights reserved.

Addresses: Nucl Desenvolvimento Farmaceut & Cosmet, BR-50740520 Recife, PE, Brazil

Univ Fed Paraiba, LTF, BR-58059900 Joao Pessoa, Paraiba, Brazil

Reprint Address: BEDOR, DCG, NUCL DESENVOLVIMENTO FARMACEUT & COSMET, RUA PROF ARTHUR SA S-N,CIDADE UNIV, BR-50740520 RECIFE, PE, BRAZIL

Author's E-mail: danilo_bedor@yahoo.com.br

--------------------------------------------------------------------------------

307

Author(s): Buah-Bassuah, PK (Buah-Bassuah, Paul K.); von Bergmann, HM (von Bergmann, Hubertus M.); Tatchie, ET (Tatchie, Ebenezer T.); Steenkamp, CM (Steenkamp, Christine M.)



Title: A portable fibre-probe ultraviolet light emitting diode (LED)-induced fluorescence detection system - art. no. 025601

Source: MEASUREMENT SCIENCE & TECHNOLOGY, 19 (2): 25601-25601 FEB 2008

Abstract:

A portable fibre-probe fluorescence detection system comprising a continuous-wave high-power ultraviolet light emitting diode (UV LED) emitting at 365 nm as excitation source, a bifurcated fibre probe with a six-around-one fibre configuration to illuminate and read from a large target area (similar to 3.6 mm(2)) and an integrated PC-coupled spectrometer has been developed. The construction, calibration and operation of the fluorescence detection system are described. Demonstrative test measurements with the system for possible inspection of different ripening stages on some batches of horticultural and agricultural products ( lemon, mandarin, banana leaf and ivy leaf) have been performed and results presented. The system is portable, comparatively low cost, easily operated and relative immune to ambient light, thus being suitable for field measurements.

Addresses: Univ Cape Coast, Dept Phys, Laser & Fibre Opt Ctr, Cape Coast, Ghana

Univ Stellenbosch, Dept Phys, Laser Res Inst, ZA-7600 Stellenbosch, South Africa

Reprint Address: BUAH-BASSUAH, PK, UNIV CAPE COAST, DEPT PHYS, LASER & FIBRE OPT CTR, CAPE COAST, GHANA

Author's E-mail: buahbass@hotmail.com

--------------------------------------------------------------------------------

308


Author(s): Cao, J (Cao, Jun); Wei, YJ (Wei, Ying-Jie); Qi, LW (Qi, Lian-Wen); Li, P (Li, Ping); Qian, ZM (Qian, Zheng-Ming); Luo, HW (Luo, Hou-Wei); Chen, J (Chen, Jun); Zhao, J (Zhao, Jing)

Title: Determination of fifteen bioactive components in Radix et Rhizoma Salviae Miltiorrhizae by high-performance liquid chromatography with ultraviolet and mass spectrometric detection

Source: BIOMEDICAL CHROMATOGRAPHY, 22 (2): 164-172 FEB 2008

Abstract:

A high-performance liquid chromatographic (HPLC) method coupled with ultraviolet (UV) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) was established for simultaneous qualitative and quantitative determination of nine phenolic acids and six diterpenoids in Radix et Rhizoma Salviae Miltiorrhizae (RRSM). The optimal chromatographic conditions were achieved on a Zorbax C-18 column by gradient elution with 0.1% (v/v) aqueous formic acid and acetonitrile as mobile phase at the flow rate of 1.0 mL/min. The detection wavelength at 281 nm was chosen to determine the 15 bioactive components, namely danshensu (1), protocatechuic acid (2), protocatechuic aldehyde (3), caffeic acid (4), rosmarinic acid (5), lithospermic acid (6), salvianolic acid B (7), salvianolic acid A (8), salvianolic acid C (9); dihydrotanshinone I (10), cryptotanshinone (11), tanshinone I (12), methylene tanshiqunone (13), tanshinone IIA (14) and miltirone (15). Additionally, LC-ESI-TOF/MS was used to make definite identification of the constituents in samples in comparison with those reference compounds. The validation of the method included tests of linearity, sensitivity, repeatability, stability and recovery. The proposed method was successfully applied to quantify the 15 components in 21 samples; significant variations were demonstrated in the contents of the samples from diverse species and origins. The developed method could be used to effectively and comprehensively evaluate the quality of RRSM for its clinical safety and efficacy. Copyright (c) 2007 John Wiley & Sons, Ltd.

Addresses: China Pharmaceut Univ, Dept Pharmacognosy, Key Lab Modern Chinese Med, Minist Educ, Nanjing 210009, Peoples R China

Reprint Address: LI, P, CHINA PHARMACEUT UNIV, DEPT PHARMACOGNOSY, KEY LAB MODERN CHINESE MED, MINIST EDUC, 24 TONGJIA LANE, NANJING 210009, PEOPLES R CHINA

Author's E-mail: Liping2004@126.com

--------------------------------------------------------------------------------

309


Author(s): Campos-Lara, M (Campos-Lara, Maria); Pinto-Almaza, R (Pinto-Almazan, Rodolfo); Oropeza, MV (Oropeza, Martha V.); Mendoza-Espinoza, JA (Mendoza-Espinoza, Jose Alberto)

Title: Optimization of a pravastatin quantification method using HPLC with ultraviolet detection in human serum for monitoring dyslipidemic patients

Source: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 31 (5): 667-674 2008

Abstract:

A high performance liquid chromatography (HPLC) method for the estimation of pravastatin in human serum samples has been developed to monitor dyslipidemic patients. The method was fully validated and validation parameters were in the linearity range 10-200ng/mL, correlation coefficient 0.99, mean recovery 0.73, quantification limit 10ng/mL, and limit of detection, 5ng/mL; this method was applied for pravastatin determination in human serum from Mexican dyslipidemic patients. Pravastatin values found for three studied patients were 73, 57, and 10ng/mL, indicating the importance of monitoring, due to the metabolic variability of these chronic patients.

Addresses: Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Secc Externa Farmacol, CINVESTAV PIN, Mexico City 07000, DF, Mexico

Inst Mexicano Seguro Social, Ctr Med Nacl SXXI, Unidad Invest Med & Farmacol, Mexico City, DF, Mexico

Reprint Address: MENDOZA-ESPINOZA, JA, INST POLITECN NACL, CTR INVEST & ESTUDIOS AVANZADOS, SECC EXTERNA FARMACOL, CINVESTAV PIN, APDO 4-740, MEXICO CITY 07000, DF, MEXICO

--------------------------------------------------------------------------------

310


Author(s): Jimenez-Diaz, I (Jimenez-Diaz, Inmaculada); Ballesteros, O (Ballesteros, Oscar); Vilchez, JL (Vilchez, Jose Luis); Navalon, A (Navalon, Alberto)

Title: Determination of sulfophenyl carboxylic acids in agricultural groundwater samples by CE with ultraviolet absorption detection

Source: ELECTROPHORESIS, 29 (2): 516-525 JAN 2008

Abstract:

An analytical method for the determination of six sulfophenyl carboxylic acids, namely (p-sulfophenyl) acetic, 2-(p-sulfophenyl)propionic, 2-(p-sulfophenyl)butyric, 3-(p-sulfophenyl)butyric, 4-(p-sulfophenyl)butyric, and 5-(p-sulfophenyl)valerianic acid, in agricultural irrigation water samples was developed. It involves an SPE procedure, an on-line pre-concentration normal stacking mode and subsequent separation and determination using CE with UV detection (CE-UV). p-Sulfobenzoic acid was used as internal standard. The compounds were separated with an uncoated capillary and a 25 mM ammonium acetate/ acetic acid buffer solution (pH 5.5) with 2-propanol (30% v/v) and 0.75 mM CTAB. Analyses were run at -25 kV, 25 degrees C, and 100 s of hydrodynamic injection with UV detection at 225 nm. Quantification limits found ranged between 4 and 6 ng/mL. The proposed method was validated using a recovery assay. It was satisfactorily used for the determination of these compounds in groundwater samples to track down the biodegradation of linear alkylbenzene sulfonates in an agricultural soil from the fertile plain of Granada (Spain).

Addresses: Univ Granada, Fac Sci, Dept Analyt Chem, Res Grp Analyt Chem & Life Sci, E-18071 Granada, Spain

Reprint Address: NAVALON, A, UNIV GRANADA, FAC SCI, DEPT ANALYT CHEM, RES GRP ANALYT CHEM & LIFE SCI, E-18071 GRANADA, SPAIN

Author's E-mail: anavalon@ugr.es

--------------------------------------------------------------------------------

311


Author(s): van der Klift, EJC (van der Klift, Elbert J. C.); Vivo-Truyols, G (Vivo-Truyols, Gabriel); Claassen, FW (Claassen, Frank W.); van Holthoon, FL (van Holthoon, Frederique L.); van Beek, TA (van Beek, Teris A.)

Title: Comprehensive two-dimensional liquid chromatography with ultraviolet, evaporative light scattering and mass spectrometric detection of triacylglycerols in corn oil

Source: JOURNAL OF CHROMATOGRAPHY A, 1178 (1-2): 43-55 JAN 12 2008

Abstract:

An improved comprehensive two-dimensional (LC x LC) HPLC system for the analysis of triacylglycerols was developed. In the first-dimension, a Ag(I)-coated cation exchanger (250 mm x 2.1 mm, 5 mu m) was employed with a gradient from 100% MeOH to 6% MeCN in MeOH at 20 mu L/min. Using a 10-way valve with two switching loops, 1min sections of the first-dimension were introduced in the second-dimension consisting of a 30 mm x 4.6 mm C18 (1.8 mu m) column with an isocratic mobile phase of methanol-methyl tert-butyl ether (70:30) at 3.0 mL/min. As the second-dimension solvent was stronger than the first-dimension solvent, focusing in the second-dimension took place, leading to better separations than in previously reported analyses in which hexane was the main constituent of the first-dimension eluent. Compounds differing by 2 in their partition number were baseline separated in the second-dimension. Detection took place by UV at 210nm, evaporative light scattering and (+)-atmospheric pressure chemical ionisation-MS with the latter giving the best results. Corn oil was investigated and 44 compounds could be detected: 34 triacylglycerols (TAGs), 8 oxygenated TAGs, and 2 TAGs containing a trans double bond. Data manipulation allowed the construction of contour plots and the automated calculation of the first- and second-dimension retention times and peak areas. Quantitative results are compared with a fatty acid methyl ester analysis, and with literature data. (c) 2007 Elsevier B.V. All rights reserved.

Addresses: Wageningen Univ, Nat Prod Chem Grp, Organ Chem Lab, NL-6703 HB Wageningen, Netherlands

Univ Amsterdam, Polymer Anal Grp, Hoff Inst Mol Sci, NL-1018 WV Amsterdam, Netherlands

Reprint Address: VAN BEEK, TA, WAGENINGEN UNIV, NAT PROD CHEM GRP, ORGAN CHEM LAB, DREIJENPL 8, NL-6703 HB WAGENINGEN, NETHERLANDS

Author's E-mail: teris.vanbeek@wur.nl

--------------------------------------------------------------------------------

312

Author(s): Campos-Lara, M (Campos-Lara, Maria); Mendoza-Espinoza, JA (Mendoza-Espinoza, Jose Alberto)



Title: Development of a selective extraction method for pravastatin quantification in tablets using HPLC with ultraviolet detection

Source: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 31 (4): 619-623 2008

Abstract:

A high performance liquid chromatography method for estimation of pravastatin in tablets has been developed. The mobile phase consisted of acetonitrile and phosphates buffer in a volume percentage ratio of 7:3 v/v, pH 2.0 and was delivered at the rate of 1mL/min and detected at 238nm, retention times were approximately 7.3min: this peak was analyzed with mass spectroscopy. The method was fully validated and validation parameters were: linearity range 10-200ng/mL, correlation coefficient 0.999, mean recovery 99%, limit of quantification 5ng/mL, and limit of detection 5ng/mL; this method can be used for a quality control assay.

Addresses: Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Secc Externa Farmacol, Mexico City 07000, DF, Mexico

IMSS, Ctr Med Nacl S21, Unidad Invest Med & Farmacol, Mexico City, DF, Mexico

Reprint Address: MENDOZA-ESPINOZA, JA, INST POLITECN NACL, CTR INVEST & ESTUDIOS AVANZADOS, SECC EXTERNA FARMACOL, APDO 4-740, MEXICO CITY 07000, DF, MEXICO

Author's E-mail: amendoza@cinvestav.mx


1   ...   11   12   13   14   15   16   17   18   19


База данных защищена авторским правом ©shkola.of.by 2016
звярнуцца да адміністрацыі

    Галоўная старонка